| UV与NTG复合诱变选育咪唑立宾高产菌 |
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| 引用本文： | 张祝兰,唐文力,杨煌建,任林英,孙菲,郑榕.UV与NTG复合诱变选育咪唑立宾高产菌[J].微生物学通报,2010,37(6):0857-0860. |
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| 作者姓名： | 张祝兰 唐文力 杨煌建 任林英 孙菲 郑榕 |
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| 作者单位： | 福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福建福州,350007 |
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| 基金项目： | 福建省科技重点项目(No. 2009R10003-3) |
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| 摘 要： | 为选育咪唑立宾的高产菌株,对咪唑立宾产生菌Eupenicillum sp.E-0509-2的孢子分别进行了紫外线照射(UV)、亚硝基胍(NTG)和UV+NTG复合诱变处理筛选突变株。结果表明试验所确定的咪唑立宾产生菌的孢子诱变适宜条件为:将孢子悬液在电磁搅拌下,经紫外线照射处理60s后,以3.0g/L NTG处理20min。经发酵筛选试验,获得了一株遗传性状稳定、高产咪唑立宾的诱变菌株Eupenicillum sp.E-UN41,其咪唑立宾产量较出发菌株提高13倍。
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| 关 键 词： | 咪唑立宾 正青霉菌 复合诱变 |
Breeding of a High Mizoribine-producing Strain by Composite Mutation with UV and NTG |
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| ZHANG Zhu-Lan,TANG Wen-Li,YANG Huang-Jian,REN Lin-Ying,SUN Fei and ZHENG Rong.Breeding of a High Mizoribine-producing Strain by Composite Mutation with UV and NTG[J].Microbiology,2010,37(6):0857-0860. |
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| Authors: | ZHANG Zhu-Lan TANG Wen-Li YANG Huang-Jian REN Lin-Ying SUN Fei and ZHENG Rong |
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| Institution: | Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou, Fu-jian 350007, China |
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| Abstract: | Eupenicillum sp. E-0509-2 was used as an original strain to carry out mutagenetic breeding adopting UV, NTG and UV + NTG, respectively. The spore suspension of E-0509-2 was treated by UV irradiation for 60 seconds, followed with 3.0 g/L NTG for 20 minuites under magnetic stirring. After further screening experiments on fermentation, a mutant Eupenicillum sp. E-UN41 which was able to produce mizoribine with high yield and could be stably passed on genetics was eventually found. The Mizoribine yield of Eupenicillum sp. E-UN41 was enhanced more than 13 times higher than that of the original strain. |
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| Keywords: | Mizoribine Eupenicillum Composite mutation |
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