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| 培养和非培养法分析冷藏鸡肉胴体中的细菌多样性 | |
| 引用本文： | 杨虎,向文良,张弛,孙烨琨,王璐,黄敏,孙群.培养和非培养法分析冷藏鸡肉胴体中的细菌多样性[J].微生物学通报,2010,37(10):1451-1456. |
| 作者姓名： | 杨虎 向文良 张弛 孙烨琨 王璐 黄敏 孙群 |
| 作者单位： | 1. 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川成都610064 2. 西华大学生物工程学院,四川成都,610039 3. 四川师范大学生命科学学院,四川成都,610068 4. 四川省原子能研究院,四川成都,610066 |
| 基金项目： | “十一五”国家科技支撑计划项目(No. 2007BAD70B00); 农业部核技术农业应用项目(No. 200803034) |
| 摘 要： | 运用纯培养和非培养法对冷藏鸡肉胴体上细菌多样性进行了对比研究。采用培养法从鸡肉胴体中初步分离到45株细菌菌株,16S rDNA-ARDRA分析得到9株代表性细菌,其16S rDNA序列系统发育分析表明,这些菌株隶属于Bacillus sp.、Shigella sp.、Pseudomonas sp.、Citrobacter sp.、Klebsiella sp.和Escherichia sp.6个属。16S rDNA-ARDRA联合PAGE和16S rDNA全序列分析的非培养法结果表明,冷藏鸡肉中细菌主要属于Acinetobacter sp.、Bacillus sp.、Acidovorax sp.、Brochothrix thermosphacta、Lactococcus garvieae和Leuconostoc lactis等16个属。非培养法揭示的细菌多样性比培养法丰富,但二者结合使用能让肉品中微生物多样性得到更全面的展示。 |
| 关 键 词： | 冷藏鸡肉 细菌 多样性 16S rDNA-ARDRA |
Bacterial Diversity in Chilled Chicken by 16S rDNA-ARDRA and Culture Dependent Approach |
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| YANG Hu,XIANG Wen-Liang,ZHANG Chi,SUN Ye-Kun,WANG Lu,HUANG Min and SUN Qun.Bacterial Diversity in Chilled Chicken by 16S rDNA-ARDRA and Culture Dependent Approach[J].Microbiology,2010,37(10):1451-1456. | |
| Authors: | YANG Hu XIANG Wen-Liang ZHANG Chi SUN Ye-Kun WANG Lu HUANG Min and SUN Qun |
| Institution: | 1. Key Laboratory of Bio-resource and Bio-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China;2. College of Bioengineering, Xihua University, Chengdu, Sichuan 610039, China;1. Key Laboratory of Bio-resource and Bio-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China;1. Key Laboratory of Bio-resource and Bio-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China;3. College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610068, China;4. Sichuan Institute of Atomic Energy, Chengdu, Sichuan 610066, China;1. Key Laboratory of Bio-resource and Bio-environment, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China |
| Abstract: | Bacterial diversity of chilled chicken was investigated by culture independent and dependent approach. A total of 45 colonies were recovered from the plate culture of chilled chicken based on morphological characteristics and further identified as 6 genera, including Bacillus sp., Shigella sp., Pseudomonas sp., Citrobacter sp., Klebsiella sp. and Escherichia sp., according to their 16S rDNA sequences. Direct 16S rDNA-ARDRA coupled with sequences analysis of DNA extracted from chilled meat showed that the bacteria could be assigned to 16 genera of Acinetobacter sp., Bacillus sp., Acidovorax sp., Brochothrix thermosphacta, Lactococcus garvieae and Leuconostoc lactis etc. Compared to culture dependent method, culture independent approach disclosed a more diversified bacterial community in chilled chicken, but culture dependent one was still a necessary supplement by showing unique species when molecular analysis was used to investigate microbial diversity. |
| Keywords: | Chilled chicken Bacteria Diversity 16S rDNA-ARDRA |
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