| 一株降氰细菌的筛选及其转化特性初步研究 |
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| 引用本文： | 刘幽燕,何玉财,李青云,韩文亮,童张法,何勇强.一株降氰细菌的筛选及其转化特性初步研究[J].微生物学通报,2005,32(2):25-28. |
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| 作者姓名： | 刘幽燕 何玉财 李青云 韩文亮 童张法 何勇强 |
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| 作者单位： | 1. 广西大学化学化工学院,南宁,530004
2. 广西大学微生物及植物遗传工程教育部重点实验室,南宁,530004 |
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| 基金项目： | 国家自然科学基金资助项目(No.39860038)，广西科学基金资助项目(No.桂科青0447003) |
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| 摘 要： | 从污染土壤中分离一株高效降氰菌株DN25，经表型分析和16SrDNA分析，初步判断为产碱杆菌(Alcaligenes sp．)。该菌株耐氰能力强，能在氰浓度达1，000mg／L的环境中生长。其生长和转化的最佳温度和pH分别为30％和8．0，10h对氰浓度为500mg／L的溶液转化率可达到99％。同时菌株也可有效转化亚铁氰化钾，对于氰浓度相当于500mg／L的亚铁氰化钾液，12h的转化率可达到96％。
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| 关 键 词： | 氰化物 生物转化 筛选 转化特性 |
| 文章编号： | 0253-2654(2005)02-0025-04 |
| 修稿时间： | 2004年6月10日 |
Studies on the Screening of a Cyanide-Degradation Strain and Its Cyanide-Transformation Characteristics |
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| LIU You-Yah,HE Yu-Cai,LI Qing-Yun,HAN Wen-Liang,TONG Zhang-Fa,HE Yong-Qiang.Studies on the Screening of a Cyanide-Degradation Strain and Its Cyanide-Transformation Characteristics[J].Microbiology,2005,32(2):25-28. |
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| Authors: | LIU You-Yah HE Yu-Cai LI Qing-Yun HAN Wen-Liang TONG Zhang-Fa HE Yong-Qiang |
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| Institution: | LIU You-Yan ** HE Yu-Cai LI Qing-Yun HAN Wen-Liang TONG Zhang-Fa HE Yong-Qiang |
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| Abstract: | A bacterial strain DN25, effective on cyanide-degradation, was isolated from contaminated soil and identified as Alcaligenes sp. on the basis of phenotype analysis and 16S rDNA sequence analysis. It showed great tolerance to the cyanide, which can grow in the medium containing 500mg CN -/L. The suitable condition for the cell growth and boitransformation was pH8.0 and 30oC and the transformation rate for 500mg CN - /L could achieve 99% in 10 h. It has also been found that the screened strain had the ability of K 4Fe(CN) 6 transformation with 96% of transformation rate at 12 h for the concentration of 500 mg CN /L. |
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| Keywords: | Cyanide Biodegradation Screen Characteristics |
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