| 原生质体诱变选育ε-聚赖氨酸高产菌株 |
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| 引用本文： | 田丰伟,程传荣,袁维涵,赵鑫,赵建新,张灏,陈卫.原生质体诱变选育ε-聚赖氨酸高产菌株[J].微生物学通报,2010,37(10):1457-1461. |
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| 作者姓名： | 田丰伟 程传荣 袁维涵 赵鑫 赵建新 张灏 陈卫 |
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| 作者单位： | 江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122 |
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| 基金项目： | 国家863计划项目(No. 2007AA10Z353); 十一五科技支撑计划项目(No. 2009BADB9B05); 国家自然科学基金项目(No. 20836003) |
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| 摘 要： | 以白色链霉菌UN2-71为出发菌株,对其原生质体进行硫酸二乙酯(DES)诱变,选育ε-聚赖氨酸高产菌株。经过试管初筛和摇瓶复筛,得到1株稳定性好的菌株D3-32,摇瓶产量达到1.56g/L,比出发菌株提高49.43%。采用2.3L发酵罐进行发酵试验,控制pH分两阶段培养后,ε-聚赖氨酸最高产量达到4.59g/L,比出发菌株提高了2.65倍。
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| 关 键 词： | ε-聚赖氨酸 白色链霉菌 原生质体 硫酸二乙酯 诱变 |
Microbial Breeding of High ε-polylysine-producing Strains by Diethyl Sulfate Protoplast Mutagenesis |
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| TIAN Feng-Wei,CHENG Chuan-Rong,YUAN Wei-Han,ZHAO Xin,ZHAO Jian-Xin,ZHANG Hao and CHEN Wei.Microbial Breeding of High ε-polylysine-producing Strains by Diethyl Sulfate Protoplast Mutagenesis[J].Microbiology,2010,37(10):1457-1461. |
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| Authors: | TIAN Feng-Wei CHENG Chuan-Rong YUAN Wei-Han ZHAO Xin ZHAO Jian-Xin ZHANG Hao and CHEN Wei |
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| Institution: | School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China;School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214122, China |
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