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微生物来源的L-阿拉伯糖异构酶的研究进展及应用前景
引用本文:程丽芳,沐万孟,张 涛,江 波.微生物来源的L-阿拉伯糖异构酶的研究进展及应用前景[J].微生物学通报,2008,35(10):1626-1632.
作者姓名:程丽芳  沐万孟  张 涛  江 波
作者单位:江南大学食品科学与技术国家重点实验室,无锡,214122
基金项目:国家高技术研究发展计划(863计划)
摘    要:L-阿拉伯糖异构酶(L-AI)能分别催化L-阿拉伯糖和D-半乳糖异构为L-核酮糖和D-塔格糖,它是目前生物法生产新型功能性因子D-塔格糖最为有效的酶.近年来,L-AI的结构已被揭晓,其基因已获得克隆、测序和过量表达,经过蛋白质工程改造的L-AI将是未来工业化生产D-塔格糖的主要用酶.本文综述了近年来国外对L-AI的结构与功能、催化机理、酶学性质及应用于D-塔格糖生产方面的研究状况,并展望了其发展前景.

关 键 词:L-阿拉伯糖异构酶  D-塔格糖  结构与功能  酶学性质  基因工程

Research Progress on the Microbial L-Arabinose Isomerase
CHENG Li-Fang,MU Wan-Meng,ZHANG Tao and JIANG Bo.Research Progress on the Microbial L-Arabinose Isomerase[J].Microbiology,2008,35(10):1626-1632.
Authors:CHENG Li-Fang  MU Wan-Meng  ZHANG Tao and JIANG Bo
Institution:State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122
Abstract:L-arabinose isomerase (L-AI) can isomerize L-arabinose and D-galactose into L-ribulose and D-tagatose, respectively, which is currently the most effective biological catalyst for D-tagatose production. The crystal structure of L-AI has been solved recently and its gene has been cloned, sequenced and overexpressed. L-AI improved by protein engineering will be the dominant enzyme for industrial production of D-tagatose. This paper reviewed researches on protein structure and function, properties and application in D-tagatose production of L-AI, and the long-term potential development of L-AI was prospected.
Keywords:L-arabinose isomerase  D-tagatose  Structure and function  Enzyme properties  Genetic engineering
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