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新疆一号冰川土壤细菌多样性的研究
引用本文:石鹏君,孟昆,伍宁丰,付建红,姚斌,李江,邵娜.新疆一号冰川土壤细菌多样性的研究[J].微生物学通报,2006,33(4):58-63.
作者姓名:石鹏君  孟昆  伍宁丰  付建红  姚斌  李江  邵娜
作者单位:1. 中国农业科学院饲料研究所,北京,100081
2. 中国农业科学院生物技术研究所,北京,100081
3. 新疆农业科学院微生物研究所,乌鲁木齐,830000
基金项目:国际科技合作重点项目计划(No.2004DFA060800)
摘    要:应用变性梯度凝胶电泳(DGGE)技术分离PCR扩增的16SrDNA来研究土壤微生物的多样性。直接从新疆一号冰川不同海拔高度的土壤样品中提取总DNA。用两套细菌通用引物分别扩增16SrDNA的V3和V6/V9高变区的特异性片段,PCR产物进行DGGE分析。PCR—DGGE图谱表明,PCR产物经DGGE检测到的条带清晰且分离效果好。结果表明,PCR—DGGE是一种快速研究微生物群落结构的有效方法。

关 键 词:冰川土壤  DGGE  细菌多样性
文章编号:0253-2654(2006)04-0058-06
收稿时间:2005-10-20
修稿时间:2005-12-28

Application of PCR-DGGE to Study Microbial Diversity in Glacier No. 1 Soils in Xinjiang
SHI Peng-Jun, MENG Kun,WU Ning-Feng, FU Jian-Hong, YAO Bin.Application of PCR-DGGE to Study Microbial Diversity in Glacier No. 1 Soils in Xinjiang[J].Microbiology,2006,33(4):58-63.
Authors:SHI Peng-Jun  MENG Kun  WU Ning-Feng  FU Jian-Hong  YAO Bin
Institution:1. Feed Research Instiute, Chinese Academy of Agricultural Sciences, Beijing 100081 ; 2.Biotechnology Research Inatitute, Chinese Academy of Agricultural Sciences, Beijing 100081 ; 3. Microbial Application Institute, Xinjiang Agriculture Sciences Academy, Urumqi 830000
Abstract:Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis(DGGE) was tested as a means to study microbial community composition in soil samples.The soil total DNA was directly extracted from five Glacier No.1 soils from different altitudes in xinjiang.Two sets of primers specific for bacteria (V3 and V6/V9 regions of 16S rDNA) were used to amplify specific segments.The PCR products were analyzed using DGGE.The electrophoresis lanes obtained by PCR-DGGE were legible and distinguishable.The results suggested that PCR-DGGE is a rapid and efficient method to discriminate among microbial communities.
Keywords:Glavier soil  DGGE  Microbial diversity
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