|
|||||
|
|
| MALDI-TOF质谱技术分析与鉴定病原细菌研究 | |
| 引用本文： | 其布勒哈斯,田世民,邹明强,贺银凤,陈 艳,赵贵明.MALDI-TOF质谱技术分析与鉴定病原细菌研究[J].微生物学通报,2009,36(3):0416-0426. |
| 作者姓名： | 其布勒哈斯 田世民 邹明强 贺银凤 陈 艳 赵贵明 |
| 作者单位： | 1. 内蒙古农业大学,内蒙古,呼和浩特,010018;中国检验检疫科学研究院,北京,100123 2. 中国检验检疫科学研究院,北京,100123 3. 内蒙古农业大学,内蒙古,呼和浩特,010018 |
| 基金项目： | “十一五”国家科技支撑计划项目(No. 2006BAK10B09); 中国检科院基本科研基金项目(No. 2007JK011) |
| 摘 要： | 本文通过基于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术分析病原细菌的方法进行研究, 阐明影响分析结果的重要因素, 并建立了MALDI-TOF-MS 分析病原细菌的标准方法。对不同属、种和亚种的12株植物病原细菌进行全细胞分析结果表明：MALDI-TOF-MS能快速而准确的区分和鉴定病原细菌, 分析过程简单、灵敏度高。此法在细菌属、种、亚种和菌株水平上, 可快速、准确地区分和鉴定。 |
| 关 键 词： | MALDI-TOF MS 细菌质量指纹图谱 鉴定 病原细菌 |
Analysis and Identification of Pathogenic Bacteria by MALDI-TOF Mass Spectrometry |
|
| Qibulehasi,TIAN Shi-Min,ZOU Ming-Qiang,HE Yin-Feng,CHEN Yan and ZHAO Gui-Ming.Analysis and Identification of Pathogenic Bacteria by MALDI-TOF Mass Spectrometry[J].Microbiology,2009,36(3):0416-0426. | |
| Authors: | Qibulehasi TIAN Shi-Min ZOU Ming-Qiang HE Yin-Feng CHEN Yan and ZHAO Gui-Ming |
| Institution: | (1. Inner Mongolian Agricultural University, Hohhot, Inner Mongolia 010018, China)(2. Chinese Academy of Inspection and Quarantine, Beijing 100123, China);Chinese Academy of Inspection and Quarantine, Beijing 100123, China;Chinese Academy of Inspection and Quarantine, Beijing 100123, China;Inner Mongolian Agricultural University, Hohhot, Inner Mongolia 010018, China;Chinese Academy of Inspection and Quarantine, Beijing 100123, China;Chinese Academy of Inspection and Quarantine, Beijing 100123, China |
| Abstract: | Based on Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI- TOF-MS) method for discrimination of pathogenic bacteria was developed. Established the standardization method for MALDI-TOF-MS analysis of bacteria and affecting elements of the analyzing result. The results obtained from twelve plants pathogenic bacteria of different genus, species and subspecies were analyzed by using MALDI-TOF showed that, MALDI-TOF-MS could be rapid and reliable identification of bacteria at the level of genus, species, and strains. Analytical process is simple, high sensitivity are advantages of this strategy. |
| Keywords: | MALDI-TOF-MS Bacteria mass finger-printing Identification Pathogenic bacteria |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《微生物学通报》浏览原始摘要信息 | |
| 点击此处可从《微生物学通报》下载免费的PDF全文 | |