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| 以淀粉为碳源衣康酸高产菌株的筛选 | |
| 引用本文： | 魏凌云,梁海秋,周河治,张佑红,丁一刚.以淀粉为碳源衣康酸高产菌株的筛选[J].微生物学通报,2010,37(2):0246-0250. |
| 作者姓名： | 魏凌云 梁海秋 周河治 张佑红 丁一刚 |
| 作者单位： | 1. 武汉工程大学绿色化工过程省部共建教育部重点实验室,湖北武汉430073;广西大学生命科学与技术学院,广西南宁530004 2. 广西大学生命科学与技术学院,广西南宁,530004 3. 武汉工程大学绿色化工过程省部共建教育部重点实验室,湖北武汉,430073 |
| 基金项目： | 湖北省教育厅科研项目(No. B20081509) |
| 摘 要： | 对土曲霉出发菌株进行紫外线诱变、LiCl诱变以及代谢终产物抗性菌株选育。代谢终产物抗性菌株选育是一种有效的遗传育种方法,能显著提高产酸量。得到一株代号为At394的菌株,以玉米淀粉部分水解糖为碳源,产酸量为53.9g/L,比出发菌株提高了42.6%。糖酸转化率为61.5%,为所有筛选菌株最高。用红外光谱进行结构分析证实所得产物为衣康酸。 |
| 关 键 词： | 衣康酸 土曲霉 玉米淀粉 筛选 |
Screening of High Itaconic Acid Yielding Strain Using Corn Starch |
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| WEI Ling-Yun,LIANG Hai-Qiu,ZHOU He-Zhi,ZHANG You-Hong and DING Yi-Gang.Screening of High Itaconic Acid Yielding Strain Using Corn Starch[J].Microbiology,2010,37(2):0246-0250. | |
| Authors: | WEI Ling-Yun LIANG Hai-Qiu ZHOU He-Zhi ZHANG You-Hong and DING Yi-Gang |
| Institution: | 1. Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan, Hubei 430073, China; 2. College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China;2. College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China;2. College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China;1. Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan, Hubei 430073, China;1. Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan, Hubei 430073, China |
| Abstract: | This paper described the screening of itaconic acid strain of Aspergillus terreus with UV-irradiation, LiCl treatment and breeding resistant strain to metabolic end product. Breeding resistant strain was an effective measure to help raise yields steadily. A strain, designated At394, producing itaconic acid with a high yield was successfully obtained. The itaconic acid concentration produced by At394 was 53.9 g/L using starch hydrolysate in shaking flasks, which was 42.6% higher than that of the parental strain. The conversion rate was 61.5%, which was the highest value found in tests. The structure determination by infrared spectrum showed the production obtained was itaconic acid. |
| Keywords: | Itaconic acid Asperigillus terrus Corn starch Screening |
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