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| 布鲁氏菌vjbR突变株的构建及其毒力表型分析 | |
| 引用本文： | 付思美,白耀霞,曲勍,徐杰,王玉飞,钟志军,于爽,王同坤,杨毅,陈燕芬,汪舟佳,杜昕颖,黄留玉,甄清,陈泽良.布鲁氏菌vjbR突变株的构建及其毒力表型分析[J].微生物学通报,2010,37(8):1188-1193. |
| 作者姓名： | 付思美 白耀霞 曲勍 徐杰 王玉飞 钟志军 于爽 王同坤 杨毅 陈燕芬 汪舟佳 杜昕颖 黄留玉 甄清 陈泽良 |
| 作者单位： | 1. 军事医学科学院疾病预防控制所,北京,100071;吉林大学公共卫生学院人畜共患病教育部重点实验室,吉林,长春,130021 2. 军事医学科学院疾病预防控制所,北京,100071 3. 吉林大学公共卫生学院人畜共患病教育部重点实验室,吉林,长春,130021 |
| 基金项目： | 国家863计划项目(No. 2007AA02Z412); 国家传染病重大专项项目(No. 2008ZX10004-015, 2009ZX10004-103) |
| 摘 要： | 为分析vjbR在布鲁氏菌毒力中的作用,构建了vjbR的突变株和互补株,并分析了它们在巨噬细胞和小鼠体内的存活能力。利用同源重组的方法,用卡那抗性基因替换了16M的vjbR(BMEII1116)基因,得到了vjbR的缺失突变株16M△vjbR。将vjbR基因的ORF克隆到pMD18-T载体中,然后将其转入到突变株16M△vjbR中得到互补株16M△vjbR-C。用16M、16M△vjbR和16M△vjbR-C侵染巨噬细胞和感染小鼠,比较分析它们在巨噬细胞内的生存能力及小鼠毒力。研究结果表明vjbR突变株在巨噬细胞和小鼠体内的毒力减弱,存活能力下降,说明vjbR基因是布鲁氏菌16M的毒力相关基因,对于布鲁氏菌建立慢性感染是必要的。 |
| 关 键 词： | 布鲁氏菌 vjbR QS系统 毒力 |
Construction of the vjbR Mutant Strain and Analysis of Its Virulence Phenotypes |
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| FU Si-Mei,BAI Yao-Xi,QU Qing,XU Jie,WANG Yu-Fei,ZHONG Zhi-Jun,YU Shuang,WANG Tong-Kun,YANG Yi,CHEN Yan-Fen,WANG Zhou-Ji,DU Xin-Ying,HUANG Liu-Yu,ZHEN Qing and CHEN Ze-Liang.Construction of the vjbR Mutant Strain and Analysis of Its Virulence Phenotypes[J].Microbiology,2010,37(8):1188-1193. | |
| Authors: | FU Si-Mei BAI Yao-Xi QU Qing XU Jie WANG Yu-Fei ZHONG Zhi-Jun YU Shuang WANG Tong-Kun YANG Yi CHEN Yan-Fen WANG Zhou-Ji DU Xin-Ying HUANG Liu-Yu ZHEN Qing and CHEN Ze-Liang |
| Institution: | 1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China; 2. School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China; 2. School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China; 2. School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China;2. School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, China;1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071, China |
| Abstract: | |
| Keywords: | Brucella vjbR quorum sensing system virulence |
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