| 大型真菌中SSR分子标记的开发与应用进展 |
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| 引用本文： | 王东东,李良秋,马连营,陈爱枫,吴清平.大型真菌中SSR分子标记的开发与应用进展[J].微生物学通报,2013,40(4):646-654. |
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| 作者姓名： | 王东东 李良秋 马连营 陈爱枫 吴清平 |
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| 作者单位： | 广东省微生物研究所广东省菌种保藏与应用重点实验室 广东省华南应用微生物重点实验室——省部共建国家重点实验室培育基地 广东广州 510070 |
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| 基金项目： | 广东省科技计划项目(No. 2011B020303004) |
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| 摘 要： | SSR分子标记是近年来普遍应用的分子标记之一。大型真菌中常用的SSR分子标记开发策略有三类,包括传统基因文库筛选法、富集文库筛选法和数据库筛选法,其中富集文库筛选法因其分离效率高而被采用较多。本文综述大型真菌中SSR分子标记的应用现状,发现相关研究多数集中于种系鉴定和遗传多样性分析,有关遗传图谱构建和辅助育种虽有涉及但研究较少。讨论了SSR分子标记在大型真菌研究中存在的问题与发展前景。
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| 关 键 词： | 微卫星 真菌 综述 |
Progress in development and applications of SSR
molecular marker in macrofungi |
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| WANG Dong-Dong,LI Liang-Qiu,MA Lian-Ying,CHEN Ai-Feng and WU Qing-Ping.Progress in development and applications of SSR
molecular marker in macrofungi[J].Microbiology,2013,40(4):646-654. |
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| Authors: | WANG Dong-Dong LI Liang-Qiu MA Lian-Ying CHEN Ai-Feng and WU Qing-Ping |
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| Institution: | Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture
Collection and Application, State Key Laboratory of Applied Microbiology (Ministry-Guangdong
Province Jointly Breeding Base), Guangzhou, Guangdong 510070, China |
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| Abstract: | The SSR (simple sequence repeats) marker has become one of the most popular molecular markers recently. There are three strategies to develop SSR marker in macrofungi, traditional library method, enriched library method and GenBank screening. Among them, enriched library method has been used more frequently due to its higher efficiency. The applications of SSR marker in macrofungi are reviewed, with the conclusion that genetic identification and genetic diversity analysis are studied commonly while the genetic map construction and assistant breeding are still to be improved. In addition, the existing problems and developing prospects of SSR marker in macrofungi are discussed. |
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| Keywords: | Microsatellite Fungi Review |
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