兼性厌氧纤维素菌的分离与系统发育分析

从四川卧龙中国保护大熊猫研究中心提供的野外放归大熊猫“祥祥”的粪样中，分离到一株产纤维素酶的兼性厌氧菌株。该菌株经初步生理生化鉴定为肠杆菌科沙雷氏菌（Serratia），命名为Serratia JF-1116。用PCR技术扩增了该菌的16S rDNA全序列，并对其进行了克隆和测序，对该序列在GenBank中的BLAST结果表明，所有与该序列高度同源的序列均为肠杆菌科的16SrDNA基因序列，选取同源性高的菌株的16SrDNA基因序列进行系统发育分析，菌株与3株Serratia聚类在一起。

The Isolation and Phylogenetic Analysis of Facultative Anaerobic Cellulase-producing Strain

A bacillus strain was isolated from the dejecta of the Giant Panda " Xiangxiang" returned to wild, afforded by China Giant Panda Protection and Research Center, Wolong Sichuan. By primary research, the strain was identified as Serratia and called as Serratia JF-1116. The 16S rDNA gene of the bacteria strain, was amplified , cloned and sequenced. BLAST of the sequence in GenBank indicated that the species with close similarity to JF-1116 were from genus Enterobacter only. The phylogenetic tree was producted from 16S rDNA of JF-1116 and other 18 Enterobacter species with the high similarity. JF-1116 was clustered with 3 strains of Serratia.