湛江红树林滩涂可培养真菌种群多样性分析

【目的】明确湛江地区红树林滩涂海洋真菌的种类及其分布,为海洋真菌的开发利用研究奠定基础。【方法】运用稀释平板法从湛江市高桥及特呈岛红树林滩涂不同的潮位带(低、中、高)和不同树种(白骨壤、桐花树、木榄、红海榄)采集淤泥样品550份,采用真菌形态学和ITS序列分析技术进行多样性研究【。结果】分离获得海洋真菌274株,共鉴定出19属39种真菌,以曲霉属Aspergillus、青霉属Penicillium和木霉属Trichoderma真菌分离频率高,为湛江红树林滩涂优势真菌种类,尤其在中潮位带真菌种类最多。此外,分离获得真菌Talaromyces helicus,为中国新记录种。【结论】湛江红树林滩涂海洋真菌的种类十分丰富,具有潜在的开发和利用前景。

Diversity of marine culturable fungal population in mangrove wetlands of Zhanjiang

Objective] The objective of this study is to define the species and distribution of the fungi in mangrove wetlands, and to lay the foundation for the development and utilization of marine fungi. Methods] Five hundred fifty soil samples were collected from different soil types of trees (Avicennia maria, Aegiceras corniculatum, Bruguiera gymnorrhiza, Rhizophora stylosa) and tidal zone (low, middle, high). The fungal isolate was isolated by dilution plate technique from the soil samples. Fungal diversity and species identification was carried out by based on the fungal morphology and rDNA ITS analysis. Results] A total of 274 fungal isolates were isolated from the soil samples. Among the 274 isolates, 39 fungal species in 19 genera were found. The dominant fungal genera within Zhangjiang mangrove tidal-flat area were Aspergillus, Penicillium and Trichoderma. In addition, the fungal species of Talaromyces helicus was a new record specie in China. Conclusion] There is a wide variety of fungal species in mangrove wetlands of Zhanjiang, and with potential prospects off development and utilization.