| 呼肠病毒BYD株吸附蛋白σ1的原核表达及其抗原性鉴定 |
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| 引用本文： | 宋立华,何君,朱虹,刘光桥,黄如统,貌盼勇,端青.呼肠病毒BYD株吸附蛋白σ1的原核表达及其抗原性鉴定[J].微生物学通报,2007,34(6):1093-1096. |
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| 作者姓名： | 宋立华 何君 朱虹 刘光桥 黄如统 貌盼勇 端青 |
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| 作者单位： | 1. 病原微生物生物安全国家重点实验室,军事医学科学院微生物流行病研究所,北京,100071
2. 解放军302医院传染病研究所病毒研究室,北京,100039 |
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| 摘 要： | 将呼肠病毒BYD株S1片段编码的细胞吸附蛋白基因连接至pET-28a( ),构建表达载体pET-28a( )-S1,转化表达宿主菌E.coli BL21(DE3),分析表达载体能否表达预期大小的重组σ1,并进一步鉴定重组σ1的抗原性。SDS-PAGE实验表明,经IPTG诱导后,表达载体能表达53kD左右蛋白质,与重组σ1的预期大小相符;免疫印迹分析表明重组σ1有较好的抗原性和特异性,可用于呼肠病毒诊断试剂的制备。
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| 关 键 词： | 呼肠病毒 S1片段 细胞吸附蛋白 原核表达 |
| 文章编号： | 0253-2654（2007）06-1093-04 |
| 收稿时间： | 2007-02-28 |
| 修稿时间： | 2007-05-08 |
Prokaryotic Expression and Antigenicity Analysis of the Attachment Protein σ1 of Reovirus BYD Strain |
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| SONG Li-Hu,HE Jun,ZHU Hong,LIU Guang-Qiao,HUANG Ru-Tong,MAO Pan-Yong and DUAN Qing.Prokaryotic Expression and Antigenicity Analysis of the Attachment Protein σ1 of Reovirus BYD Strain[J].Microbiology,2007,34(6):1093-1096. |
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| Authors: | SONG Li-Hu HE Jun ZHU Hong LIU Guang-Qiao HUANG Ru-Tong MAO Pan-Yong and DUAN Qing |
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| Institution: | State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071;Department of Virology, the 302nd Hospital of PLA, Beijing 100039;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071 |
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| Abstract: | |
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| Keywords: | Reovirus S1 segment Cell attachment protein Prokaryotic expression |
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