| 一株分离于酸性矿坑水的异养菌的特性与分子鉴定 |
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| 引用本文： | 高健,蒋莹,吴学玲,邱冠周.一株分离于酸性矿坑水的异养菌的特性与分子鉴定[J].微生物学通报,2007,34(5):0921-0925. |
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| 作者姓名： | 高健 蒋莹 吴学玲 邱冠周 |
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| 作者单位： | 1. 中南大学资源加工与生物工程学院,长沙,410083;湖南科技大学生命科学学院,湘潭,410201
2. 中南大学资源加工与生物工程学院,长沙,410083 |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划) |
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| 摘 要： | 采用平板划线分离法,从江西某铜矿酸性矿坑水中分离出一株极端嗜酸、又能耐受中度碱性条件的异养微生物,命名为HJM菌株。该菌株能在pH1.5~10.0的范围内生长。形态学以及18SrDNA和26S rDNAD1/D2区序列分析表明,HJM菌株属于P.guilliermondii这个种。金属抗性试验表明,该菌株对重金属铜离子的抗性可高达45mmol/L,因此,它的分离为研究极端环境中微生物的抗铜机制提供了材料。
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| 关 键 词： | 嗜酸 耐碱 金属抗性 序列分析 |
| 文章编号： | 0253-2654（2007）05-0921-05 |
| 修稿时间： | 2006-12-28 |
Characteristics and Molecular Identification of a Strain of Heterotrophic Microorganism Isolated from Acid Mine Drainage |
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| GAO Jian,JIANG Ying,WU Xue-Ling and QIU Guan-Zhou.Characteristics and Molecular Identification of a Strain of Heterotrophic Microorganism Isolated from Acid Mine Drainage[J].Microbiology,2007,34(5):0921-0925. |
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| Authors: | GAO Jian JIANG Ying WU Xue-Ling and QIU Guan-Zhou |
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| Institution: | (1.School of Minerals Processing and Bioengineering, Central South University, Changsha 410083) (2.School of Life Science, Hunan University of Science and Technology, Xiangtan 410201);School of Minerals Processing and Bioengineering, Central South University, Changsha 410083;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083 |
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| Abstract: | By streak plate method, a strain of heterotrophic microorganism which was extremely acidophilic, alkalitolerant, named by HIM, was isolated from an acid mine drainage of a certain copper mineral in Jiangxi Province. This strain was able to grow under pH value of 1.5 - 10.0. Morphological characteristics and sequences analysis of 16S rDNA and 26S rDNA D1/D2 region revealed that it belonged to the species P. guilliermondii. Resistant experiment to metals showed that it could resist copper of 45mmol/L, so it was an important strain used to study the resistance mechanism of copper. |
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| Keywords: | P guilliermondii |
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