| 黄曲霉和米曲霉的多相鉴定方法 |
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| 引用本文： | 白飞荣,姚粟,凌空,程池.黄曲霉和米曲霉的多相鉴定方法[J].微生物学通报,2018,45(1):215-226. |
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| 作者姓名： | 白飞荣 姚粟 凌空 程池 |
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| 作者单位： | 中国食品发酵工业研究院中国工业微生物菌种保藏管理中心 北京 100015,中国食品发酵工业研究院中国工业微生物菌种保藏管理中心 北京 100015,中国食品发酵工业研究院中国工业微生物菌种保藏管理中心 北京 100015,中国食品发酵工业研究院中国工业微生物菌种保藏管理中心 北京 100015 |
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| 基金项目： | 国家重点研发计划(2016YFD0400500) |
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| 摘 要： | 【背景】黄曲霉(Aspergillus flavus)和米曲霉(Aspergillus oryzae)形态特征相近,基因组高度相似,较难区分。【目的】旨在总结一套准确鉴别二者的分类方法。【方法】利用22株标准菌株对传统形态学、产毒培养基、酶联免疫毒素检测、系统发育分析、产毒基因检测等5种鉴别方法分别进行验证。【结果】各鉴定方法的结果存在异同,单一的鉴定方法容易出现假阴性或假阳性结果。【结论】利用单一方法区分黄曲霉和米曲霉具有潜在风险,多相鉴定方法可以准确鉴别二者。
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| 关 键 词： | 黄曲霉，米曲霉，多相鉴定 |
The polyphasic taxonomy for distinguishing Aspergillus flavus and A. oryzae |
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| BAI Fei-Rong,YAO Su,LING Kong and CHENG Chi.The polyphasic taxonomy for distinguishing Aspergillus flavus and A. oryzae[J].Microbiology,2018,45(1):215-226. |
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| Authors: | BAI Fei-Rong YAO Su LING Kong and CHENG Chi |
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| Institution: | China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China,China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China,China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China and China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China |
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| Abstract: | Background] The morphology and genome between Aspergillus flavus and A. oryzae were highly similar, which made it very difficult to distinguish with each other. Objective] The aim of this study was to establish an accurate method for distinguishing A. flavus with A. oryzae. Methods] Twenty-two standard strains were used to study the five traditional identification methods of A. flavus and A. oryzae, including the traditional morphological identification method, enzyme linked immunosorbent assay (ELISA), the toxin-secreting culture medium method, the phylogenetic tree method and the detection method for toxin genes. Results] There are differences or complements among the results of different methods. Conclusion] A single method used to distinguish the two species would have a potential risk, while a polyphasic approach could resolve this problem. |
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| Keywords: | Aspergillus flavus Aspergillus oryzae Polyphasic taxonomy |
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