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| 产氢产乙酸菌ZR-1 的分离鉴定及产酸特性 | |
| 引用本文： | 习彦花,程辉彩,张丽萍,崔冠慧,马金亮.产氢产乙酸菌ZR-1 的分离鉴定及产酸特性[J].微生物学通报,2011,38(2):181-186. |
| 作者姓名： | 习彦花 程辉彩 张丽萍 崔冠慧 马金亮 |
| 作者单位： | 1. 河北省科学院生物研究所,河北,石家庄,050081;河北师范大学生命科学学院,河北,石家庄,050016 2. 河北省科学院生物研究所,河北,石家庄,050081 3. 河北省科学院生物研究所,河北,石家庄,050081;河北大学生命科学学院,河北,保定,071002 |
| 基金项目： | 国家“十一五”科技支撑项目(No. 2008BADCC4B02-8); 河北省科技支撑项目(No. 08225502D) |
| 摘 要： | 采用改良的亨盖特厌氧操作技术, 从有机废水污泥中分离到一株耐低温高效产氢产乙酸菌ZR-1。经过对其形态学观察、生理生化特征研究及16S rRNA 序列比对, 初步鉴定为梭状芽胞杆菌属的乙二醇梭菌(Clostridium glycolicum)。通过单因子实验, 在厌氧条件下对该菌株的培养温度、pH、最适底物、金属离子的影响等产酸条件进行了优化。结果表明该菌株最适生长温度37 °C,最佳培养基初始pH 值8.5, 最适发酵底物丁酸盐, Mn2+对其产酸有一定的激活作用。最适培养条件下丁酸盐降解率达到12.7%, H2 含量达到了28.73%。 |
| 关 键 词： | 厌氧消化 产氢产乙酸菌 鉴定 生酸特性 |
| 收稿时间： | 2010/7/12 0:00:00 |
| 修稿时间： | 2010/11/29 0:00:00 |
Separation and identification of a hydrogen-producing acetogens strain ZR-1 and its acetic production characters |
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| XI Yan-Hu,CHENG Hui-Cai,ZHANG Li-Ping,CUI Guan-Hui and MA Jin-Liang.Separation and identification of a hydrogen-producing acetogens strain ZR-1 and its acetic production characters[J].Microbiology,2011,38(2):181-186. | |
| Authors: | XI Yan-Hu CHENG Hui-Cai ZHANG Li-Ping CUI Guan-Hui and MA Jin-Liang |
| Institution: | Institute of Biology of Hebei Academy of Sciences, Shijiazhuang, Hebei 050081, China; College of Life Sciences of Hebei Nomal University, Shijiazhuang, Hebei 050016, China;Institute of Biology of Hebei Academy of Sciences, Shijiazhuang, Hebei 050081, China;Institute of Biology of Hebei Academy of Sciences, Shijiazhuang, Hebei 050081, China;Institute of Biology of Hebei Academy of Sciences, Shijiazhuang, Hebei 050081, China;Institute of Biology of Hebei Academy of Sciences, Shijiazhuang, Hebei 050081, China; College of Life Sciences of Hebei University, Baoding, Hebei 071002, China |
| Abstract: | A hydrogen-producing acetogen strain ZR-1 was isolated from organic wastewater sludge by applying modified Hungate anaerobic method.According to morphological observation,physiological-biochemical characteristics and homology analysis of 16S rRNA gene sequence,the strain was identified as a genus of Clostridium glycolicum.The effects of temperature,pH,optimum substrate and metal ions on its productivity of acetic acid in anaerobic conditions were investigated and then optimized by uniform tests.The results ... |
| Keywords: | Anaerobic digestion Hydrogen-producing acetogen Identify Acetic acid production characteristics |
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