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海水养殖场沉积物中硝酸盐还原菌种群分析
引用本文:王亚南, 王保军, 戴欣, 焦念志, 彭志英, 刘双江,.海水养殖场沉积物中硝酸盐还原菌种群分析[J].微生物学通报,2004,31(6):73-76.
作者姓名:王亚南  王保军  戴欣  焦念志  彭志英  刘双江  
作者单位:1. 中国科学院微生物研究所,北京,100080;华南理工大学食品与生物工程学院,广州,510641
2. 中国科学院微生物研究所,北京,100080
3. 厦门大学海洋环境科学系,厦门,361000
4. 华南理工大学食品与生物工程学院,广州,510641
基金项目:本项研究得到了中国科学院知识创新工程项目(KZCX1-SW-12-Ⅱ)和百人计划的支持.
摘    要:通过对福建省沿海海水养殖场沉积物中参与氮循环的各生理群细菌数量分析 ,发现氨化和硝酸盐还原细菌是优势生理菌群 ,同时 ,表层泥样中的硝酸盐还原菌数量明显高于深层泥样。从该环境中分离获得 106株细菌 ,其中 58株具有硝酸盐还原能力 ,初步鉴定表明它们主要为芽孢杆菌属 (Bacillus)、盐芽孢杆菌属 (Halobacillus)、短芽孢杆菌属 (Brevibacil lus)、动性球菌属 (Planococcus)和动性杆菌属 (Plano

关 键 词:海水养殖    沉积物    氮循环    硝酸盐还原菌  
文章编号:0253-2654(2004)06-0073-04
修稿时间:2004年2月23日

Analysis of Nitrate Reducing Community in a Near-shore Marine-cultural Sediments
WANG Ya-Nan , WANG Bao-Jun DAI Xin JIAO Nian-Zhi PENG Zhi-Ying LIU Shuang-Jiang.Analysis of Nitrate Reducing Community in a Near-shore Marine-cultural Sediments[J].Microbiology,2004,31(6):73-76.
Authors:WANG Ya-Nan  WANG Bao-Jun DAI Xin JIAO Nian-Zhi PENG Zhi-Ying LIU Shuang-Jiang
Institution:WANG Ya-Nan 1,2 WANG Bao-Jun 1 DAI Xin 1 JIAO Nian-Zhi 3 PENG Zhi-Ying 2 LIU Shuang-Jiang 1*
Abstract:The nitrogen-cycling bacteria in a near-shore marine-cultural sediments were investigated. Results indicated that ammonifying and nitrate-reducing bacteria (NRB) were the most abounding populations. The abundance of nitrate-reducing bacteria occurred in the surface layer of the sediment was higher than that in the bottom layer. 106 bacterial strains were obtained from sediment samples and were tested for their nitrate reducing ability. The results showed that 58 strains of them were able to reduce nitrate. The dominant nitrate-reducing strains were preliminarily identified as Gram positive bacteria and belong to the genus of Bacillus, Halobacillus, Brevibacillus, Planococcus and Planomicrobium.The richness of diversity of nitrate-reducing bacteria was further revealed by the analysis of the sequences of their 16S rRNA genes.
Keywords:Marine-culture  Sediment  Nitrogen-cycling  Nitrate-reducing bacteria
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