| 用抗性筛选法选育γ—亚麻酸（GLA）高产菌株 |
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| 引用本文： | 陈波,张玲,等.用抗性筛选法选育γ—亚麻酸（GLA）高产菌株[J].微生物学通报,2003,30(1):53-56. |
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| 作者姓名： | 陈波 张玲 |
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| 作者单位： | 西南科技大学生命科学与工程学院，绵阳621000 |
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| 摘 要： | 以深黄被孢霉（Mortierella isabellina）为出发菌株，经紫外线诱变处理，采用抗性筛选法，直接在梯度平板上挑选取抗脂肪酸脱氢酶抑制物抑芽丹（maleic hydrazide）的菌株进行初筛，然后经摇瓶发酵法测定相关性能指标进行得筛，获得一株生产性能比出发菌株显提高的突变株M80，其菌体收率达25.10g/L、油脂产率达12.35g/L、γ-亚麻酸（GLA）产率达771.88mg/L。
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| 关 键 词： | 深黄被孢霉 紫外线诱变 抗性筛选 突变株 |
| 文章编号： | 0253-2654(2003)01-0053-04 |
| 修稿时间： | 2001年12月4日 |
SCREENING OF MUTANT OVERPRODUCING GLA WITH RESISTANCE METHOD |
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| EN Bo,ZHANG Ling,HE Xin Sheng,LI Dai Fa,WANG Xi.SCREENING OF MUTANT OVERPRODUCING GLA WITH RESISTANCE METHOD[J].Microbiology,2003,30(1):53-56. |
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| Authors: | EN Bo ZHANG Ling HE Xin Sheng LI Dai Fa WANG Xi |
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| Abstract: | Using Mortierella isabellina as starting strain, mutagenized with UV,screened by selecting mutants resisting maleic hydrazide,a fatty acid dehydrogenase inhibitor, directly on gradient plate,a mutant M 80 overproducing Gamma Linolenic Acid (GLA) was obtained, which has much higher productive qualities than its parental strain Its dry cell weight reaches 25 10g/L,lipid yield 12 35g/L,and GLA yield 771 88mg/L |
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| Keywords: | Mortierella isabellina UV mutagenesis Resistance screen Mutant |
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