绿木霉Gv29-8丝氨酸蛋白酶S8/S53超家族基因特性及功能

【背景】丝氨酸蛋白酶在木霉菌生物防治过程中发挥重要作用。【目的】研究绿木霉丝氨酸蛋白酶S8/S53超家族基因信息及其生物学功能,进而为该蛋白酶生防制剂的开发及基因改造提供理论支持。【方法】通过生物信息学分析方法,从绿木霉Gv29-8基因组中鉴定出23个丝氨酸蛋白酶基因,以少孢节丛孢菌ATCC 24927基因组中鉴定的4个丝氨酸蛋白酶基因作为对照,对这27个丝氨酸蛋白酶基因的特性、蛋白结构、进化地位、功能等进行预测分析。【结果】27个基因结构差异较大,编码的蛋白具有典型的丝氨酸蛋白酶催化三联体结构,属于S8/S53超家族,分为6个亚家族,同一亚家族的蛋白酶保守区长度相近,相似性较高,催化残基附近序列比较保守。系统进化分析显示,同一亚家族丝氨酸蛋白酶聚为一类。【结论】绿木霉和少孢节丛孢菌的部分丝氨酸蛋白酶基因在结构和蛋白性质上相似性强,亲缘关系较近,均属于S8_PCSK9_ProteinaseK_like亚家族,推测绿木霉与少孢节丛孢菌该亚家族的丝氨酸蛋白酶具有相似的功能,可抑制植物病原真菌和降解线虫体壁。

Characterization of S8/S53 serine protease superfamily gene of Trichoderma virens Gv29-8

Background] Serine protease plays an important role in the biological control of Trichoderma spp. Objective] This study provided theoretical support for the development of the protease biocontrol preparation and genetic modification by studying the serine protease S8/S53 superfamily gene information and biological functions of Trichoderma virens. Methods] Twenty-three serine protease genes were identified from T. virens Gv29-8 genome by bioinformatics analysis. Four serine protease genes identified from Arthrobotrys oligospora ATCC 24927 genome were used as controls. The characteristics, protein structure, evolutionary status and function of 27 serine protease genes were predicted and analyzed. Results] The 27 genes were significantly different in structure, and the encoded proteins had typical serine protease catalytic triad structure, belonging to the S8/S53 superfamily, which were divided into 6 subfamilies. The conserved domain of proteases in the same subfamily was similar in length, with high similarity and conservative sequences near the catalytic residues. Phylogenetic analysis showed that the serine proteases of the same subfamily were clustered into one group. Conclusion] Some serine protease genes of T. virens and A. oligospora have strong similarities in structure and protein properties and close genetic relationship, both belonging to the S8_PCSK9_ProteinaseK_like subfamily. It is speculated that T. virens and A. oligospora have similar functions of serine protease, which can inhibit plant pathogenic fungi and degrade the body wall of nematodes.