黄孢原毛平革菌BKMF-1767产絮凝剂PCF-1767的絮凝特性及其机理解析 Flocculation characteristics and mechanism of flocculant PCF-1767 produced by Phanerochaete chrysosporium BKMF-1767期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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黄孢原毛平革菌BKMF-1767产絮凝剂PCF-1767的絮凝特性及其机理解析

引用本文：	李宁杰,兰琪,陈中维,刘洁,吴磊,王敦球,张浩,黄小霞,潘莉,陈一聪,覃业维.黄孢原毛平革菌BKMF-1767产絮凝剂PCF-1767的絮凝特性及其机理解析[J].微生物学通报,2020,47(2):431-439.

作者姓名：	李宁杰兰琪陈中维刘洁吴磊王敦球张浩黄小霞潘莉陈一聪覃业维

作者单位：	1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004,1 桂林理工大学环境科学与工程学院广西桂林 541004;2 桂林理工大学广西环境污染控制理论与技术重点实验室广西桂林 541004

基金项目：	国家自然科学基金(51608142，51638006)；广西科技计划项目(桂科AD19110151，2016GXNSFBA380076，2018GXNSFGA281001，桂科AD18126018)；广西高校中青年教师基础能力提升项目(2018KY0259)

摘要：	【背景】传统絮凝剂的使用会带来安全和环境污染方面的问题,而微生物絮凝剂具有无毒、无二次污染、易生物降解的优点,因此寻找高效廉价的微生物絮凝剂具有重要意义。【目的】研究黄孢原毛平革菌BKMF-1767产生的胞外多糖絮凝剂的絮凝特性及机理。【方法】使用高岭土进行PCF-1767的絮凝活性测定,分析絮凝剂获取时间、Ca2+浓度、投加量、pH、温度对絮凝效率的影响,并测定PCF-1767的热稳定性。测定絮凝剂的单糖组成、糖苷键连接类型、多糖分子量、Zeta电位变化、对絮体形态观察,探讨了PCF-1767的絮凝机理。【结果】培养6–12 d获得的PCF-1767的絮凝活性良好;7 d絮凝剂要获得较好的絮凝效率至少需加入2 mmol/L Ca2+作为助凝剂,最佳投加浓度为0.75–1.35 mg/L,最适pH为3.0-9.0,对温度高于50°C的废水絮凝活性逐渐降低,经过沸水浴处理3 h后的絮凝活性几乎不变。PCF-1767中多糖的一级结构主要由葡萄糖以→4)Glup(1→的方式连接,多糖分子量随培养时间的延长而增大,变化范围为9.897×105–2.126×106 Da。结合Zeta电位变化与絮体显微图像分析表明其絮凝机理主要为吸附架桥和卷扫作用,而非电荷中和作用。【结论】PCF-1767具有很高絮凝活性,其用量低、适用条件广、热稳定性高,是一种非常具有应用前景的絮凝剂。
关键词：	微生物絮凝剂，胞外多糖，黄孢原毛平革菌，絮凝机理
Flocculation characteristics and mechanism of flocculant PCF-1767 produced by Phanerochaete chrysosporium BKMF-1767

LI Ning-Jie,LAN Qi,CHEN Zhong-Wei,LIU Jie,WU Lei,WANG Dun-Qiu,ZHANG Hao,HUANG Xiao-Xi,PAN Li,CHEN Yi-Cong and QIN Ye-Wei.Flocculation characteristics and mechanism of flocculant PCF-1767 produced by Phanerochaete chrysosporium BKMF-1767[J].Microbiology,2020,47(2):431-439.

Authors:	LI Ning-Jie LAN Qi CHEN Zhong-Wei LIU Jie WU Lei WANG Dun-Qiu ZHANG Hao HUANG Xiao-Xi PAN Li CHEN Yi-Cong and QIN Ye-Wei

Institution:	1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China,1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China and 1 College of Environmental Science and Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China;2 Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, China

Abstract:

Keywords:	Microbial flocculant Extracellular polysaccharide Phanerochaete chrysosporium Flocculation mechanism
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