| 几株解纤维素生防芽孢杆菌的分子鉴定及其抗逆促生特性分析 |
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| 引用本文： | 谢永丽,Renato D’Ovidio,Stefania Masci,范晶,李云龙.几株解纤维素生防芽孢杆菌的分子鉴定及其抗逆促生特性分析[J].微生物学通报,2017,44(2):348-357. |
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| 作者姓名： | 谢永丽 Renato D’Ovidio Stefania Masci 范晶 李云龙 |
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| 作者单位： | 1. 青海大学农牧学院 高原草地资源与生态省部共建重点实验室 青海 西宁 810016,2. Faculty of Agriculture, University of Tuscia, Laboratory of Agricultural biology and Agricultural chemistry, Viterbo, Itally 01100, Italy,2. Faculty of Agriculture, University of Tuscia, Laboratory of Agricultural biology and Agricultural chemistry, Viterbo, Itally 01100, Italy,1. 青海大学农牧学院 高原草地资源与生态省部共建重点实验室 青海 西宁 810016,1. 青海大学农牧学院 高原草地资源与生态省部共建重点实验室 青海 西宁 810016 |
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| 基金项目： | 青海省国际科技合作计划项目(No. 2013-H-805)；青海省(应用)基础研究计划项目(No. 2015-ZJ-732) |
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| 摘 要： | 【目的】探究青藏高原解纤维素生防芽孢杆菌资源。【方法】采用BOX-PCR指纹图谱分析、gyr B基因及16S r RNA基因序列分析,对分离自青海互助北山桦树根围的5株芽孢杆菌进行分子鉴定;通过CMC法检测菌株降解纤维素活性;平板对峙法检测菌株拮抗病原真菌及病原细菌活性;检测菌株的耐盐性、低温适生性;测定菌株促种子萌发及幼苗生长特性。【结果】5株菌均鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens;具有显著降解纤维素的特性,在CMC平板上形成纤维素降解透明圈直径≥20 mm;对油菜菌核病菌(Sclerotinia sclerotiorum)、瓜类枯萎病菌(Fusarium oxysporum)、水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)及植物梨火疫病菌(Erwinia amylovora)均有显著拮抗活性;菌株可在含Na Cl浓度为11%的LB平板上正常生长,在10°C低温条件下生长良好;菌株BS11、BS12菌悬液(细胞浓度106 CFU/m L)可促进水稻种子萌发及幼苗生长。【结论】5株B.amyloliquefaciens菌株为抗逆性强的、具有降解纤维素活性的生防芽孢杆菌,在青藏高原生态农业及畜牧产业中具有应用潜力。
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| 关 键 词： | 解纤维素活性，芽孢杆菌，拮抗活性，分子鉴定，促生及抗逆性 |
Molecular identification of cellulose-degrading bio-control Bacillus strains and their stress-resistance and growth-promoting characteristics |
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| Institution: | 1. College of Agricultural and Animal Husbandry, Qinghai University, Key Laboratory of Altiplano Grassland Resource and Ecology, Province and Education Ministry, Xining, Qinghai 810016, China,2. Faculty of Agriculture, University of Tuscia, Laboratory of Agricultural biology and Agricultural chemistry, Viterbo, Itally 01100, Italy,2. Faculty of Agriculture, University of Tuscia, Laboratory of Agricultural biology and Agricultural chemistry, Viterbo, Itally 01100, Italy,1. College of Agricultural and Animal Husbandry, Qinghai University, Key Laboratory of Altiplano Grassland Resource and Ecology, Province and Education Ministry, Xining, Qinghai 810016, China and 1. College of Agricultural and Animal Husbandry, Qinghai University, Key Laboratory of Altiplano Grassland Resource and Ecology, Province and Education Ministry, Xining, Qinghai 810016, China |
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| Abstract: | |
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| Keywords: | Cellulose-degrading activity Bacillus spp Antagonistic activity Molecular identification Growth-promoting and stress-resistance |
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