| 一株沙门氏菌拮抗菌的筛选及其在污染土壤原位修复中的应用 |
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| 引用本文： | 宋德显,尹睿,林先贵,王一明,张华勇,王纪杰.一株沙门氏菌拮抗菌的筛选及其在污染土壤原位修复中的应用[J].微生物学通报,2010,37(1):0030-0036. |
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| 作者姓名： | 宋德显 尹睿 林先贵 王一明 张华勇 王纪杰 |
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| 作者单位： | 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏南京,210008;中国科学院研究生院,北京,100049
2. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏南京,210008 |
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| 基金项目： | 国家863计划项目(No. 2006AA06Z352) |
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| 摘 要： | 利用无菌滤纸片平板法从沙门氏菌(Salmonella)污染土壤中筛选到一株有效拮抗沙门氏菌的细菌A45,通过形态学、革兰氏染色和16SrDNA序列同源性分析鉴定为产碱杆菌(Alcaligenes sp.)。温室土培试验和田间原位试验结果都发现,利用该菌株制备的沙门氏菌拮抗菌剂能显著降低土壤中沙门氏菌数量(P0.05),与对照相比土壤中沙门氏菌数量下降2-3个数量级,表明该拮抗细菌可应用于沙门氏菌污染土壤的修复。
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| 关 键 词： | 沙门氏菌 拮抗菌 土壤污染 微生物修复 产碱菌属 |
Screening of an Antagonistic Bacterial Strain Against Salmonella and Its Application in In-site Bioremediation of Salmonella-contaminated Soils |
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| SONG De-Xian,YIN Rui,LIN Xian-Gui,WANG Yi-Ming,ZHANG Hua-Yong and WANG Ji-Jie.Screening of an Antagonistic Bacterial Strain Against Salmonella and Its Application in In-site Bioremediation of Salmonella-contaminated Soils[J].Microbiology,2010,37(1):0030-0036. |
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| Authors: | SONG De-Xian YIN Rui LIN Xian-Gui WANG Yi-Ming ZHANG Hua-Yong and WANG Ji-Jie |
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| Abstract: | A bacterial strain, A45, with antagonistic activity against Salmonella was isolated from Salmonella-contaminated soils, which was identified as Alcaligenes sp. by checking the individual morphology, colony characteristics and 16S rDNA sequencing. The inhibiting effects of A45 to Salmonella in soils were investigated by field and greenhouse pot experiments respectively. The amounts of Salmonella in soils decreased up to three orders of magnitude after spraying A45 into soils both under greenhouse and field conditions. It suggested that it might be an effective, economical and environmentally friendly approach to apply A45 for in-site remediation of pathogens-contaminated soils. |
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| Keywords: | Salmonella Antagonistic bacterium Soil pollution Bioremediation Alcaligenes sp |
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