真菌生物量指示剂麦角固醇的分离及测定方法

麦角固醇作为真菌细胞膜的重要组成成分，结构稳定，可作为真菌生长的指示物质。综述了表征真菌生物量的麦角固醇的分离及测定方法，其中麦角固醇的萃取方法有传统的皂化回流法、快速物理萃取、超声波萃取、超临界流体萃取等，测定方法有高效液相色谱法、气相色谱-质谱法、液相色谱-质谱法、薄层色谱法等。并对这些方法的应用及在堆肥过程中运用麦角固醇估计真菌生物量的可行性进行了展望。

The Techniques of Isolation and Determination of Ergosterol as the Indicator of Fungal Biomass

Ergosterol is the important component of the fungal membrane, and having stable structure. This makes it a suitable indicator for growth of fungi. In the paper, isolation and determination techniques of ergosterol as the indicator of the fungal biomass were reviewed. The methods of extracting ergosterol include traditional saponification and refluxing, rapid physical disruption, rapid uhrasonication, supercfitical fluid extraction and so on. The ergosterol determination methods are high performance liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and thin-layer chromatography, et al. The application of these techniques was also introduced. Finally, the paper prospected the feasibility of applying the ergosterol as the indicator of fungal biomass in composting.