| 氯嘧磺隆降解菌株LW-3的分离及生物学特性研究 |
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| 引用本文： | 王 哲,孙纪全,马吉平,黄 星,李顺鹏.氯嘧磺隆降解菌株LW-3的分离及生物学特性研究[J].微生物学通报,2008,35(12):1899-1904. |
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| 作者姓名： | 王 哲 孙纪全 马吉平 黄 星 李顺鹏 |
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| 作者单位： | 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 农业微生物学菌种资源标准化整理、整合及其共享试点(No.2005DKA2120-2):国家863项目，国家支撑计划，江苏省社会发展项目 |
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| 摘 要： | 从长期受氯嘧磺隆污染的土壤中分离到一株氯嘧磺隆高效降解菌株,命名为LW-3,菌株LW-3可以氯嘧磺隆为唯一氮源生长,接种量为2%时,50 mg/L氯嘧磺隆经过7 d,降解率达70%～80%.生理生化实验和16S rDNA序列同源性分析,将菌株LW-3归属于假单胞茵属(Pseudomonas sp.);菌株的适宜降解条件为;温度30℃～35℃,pH 6.5～7.2,pH对菌株LW-3降解氯嘧磺隆有明显地影响.
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| 关 键 词： | 氯嘧磺隆 微生物降解 假单胞茵属 生物学特性 |
Isolation of Strain LW-3 Capable of Degrading Chlorimuron-ethyl and Its Biological Characteristics |
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| WANG Zhe,SUN Ji-Quan,MA Ji-Ping,HUANG Xing and LI Shun-Peng.Isolation of Strain LW-3 Capable of Degrading Chlorimuron-ethyl and Its Biological Characteristics[J].Microbiology,2008,35(12):1899-1904. |
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| Authors: | WANG Zhe SUN Ji-Quan MA Ji-Ping HUANG Xing and LI Shun-Peng |
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| Institution: | College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095;College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095;College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095;College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095;College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | A bacterium(designated as LW-3), capable of degrading Chlorimuron-ethyl, was isolated from the long-term contaminated soil by Chlorimuron-ethyl. Based on physiological and biochemical analyses and sequences comparison of 16S rDNA, strain LW-3 was identified as Pseudomonas sp. LW-3 could use Chlorimuron-ethyl as sole nitrogen source for growth. The optimum pH and temperature for degradation of Chlorimuron-ethyl were pH 6.5~7.2 and 30°C~35°C, at same temperature the pH change to the Chlorimuron -ethyl degrading influence is large. When the pH and temperature were pH 6.5 and 32°C, 50 mg/L Chlorimuron-ethyl could be degraded to 70%~80% level within 7 days. |
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| Keywords: | Chlorimuron-ethyl Micro-biodegradation Pseudomonas sp Biological characteristics |
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