| 农用抗生素2-16高产菌株选育及发酵优化组合研究 |
| |
| 引用本文： | 王晓青,曾洪梅,石义萍.农用抗生素2-16高产菌株选育及发酵优化组合研究[J].微生物学通报,2005,32(6):7-11. |
| |
| 作者姓名： | 王晓青 曾洪梅 石义萍 |
| |
| 作者单位： | 中国农业科学院植物保护研究所,北京,100094 |
| |
| 基金项目： | 通讯作者,Tel：86-010-62815942,Fax：86-010-62815942,E-mail：zenghongmei@hotmail.com. |
| |
| 摘 要： | 对农用抗生素2-16产生菌（Streptomyces ahygroscopicus var．huangshanensis）依次进行紫外线诱变、NTG诱变、低能碳离子注入处理，筛选获得高产菌株515号，效价较出发菌株0号提高223．10％。利用SAS软件提供的Plackett—Burman设计和响应面分析法对菌株515号的发酵培养基进行优化，采用优化培养基后效价较原始培养基提高38．53％。
|
| 关 键 词： | 农用抗生素2-16 诱变 SAS软件 培养基优化 |
| 文章编号： | 0253-2654（2005）06-0007-05 |
| 收稿时间： | 01 13 2005 12:00AM |
| 修稿时间： | 2005-01-132005-02-01 |
Selection of High-yield Agro-antibiotic 2-16 Producing Strain and Optimization of Its Cultivation |
| |
| WANG Xiao-Qing,ZENG Hong-Mei,SHI Yi-Ping.Selection of High-yield Agro-antibiotic 2-16 Producing Strain and Optimization of Its Cultivation[J].Microbiology,2005,32(6):7-11. |
| |
| Authors: | WANG Xiao-Qing ZENG Hong-Mei SHI Yi-Ping |
| |
| Institution: | Institute of Plant protection, Chinese Academy of Agriculture Sciences, Beijing 100094 |
| |
| Abstract: | In order to improve the production of agro-antibiotic 2-16,the producing strain(Streptomyces ahygroscopicus var.huangshanensis) was treated by protoplast regeneration,ultraviolet radiation,NTG mutagenesis and low energy C~( ) ion implantation.At last,a high-yield strain No.515 was obtained.The production of ~()No.515 was increased by 223.10%.By using Plackett-Burman design and Response Surface Analysis provided by SAS software,the cultivation condition of No.515 was optimized.The amount of agro-antibiotic 2-16 was increased by 38.53% when the strain No.515 was cultivated in the optimum medium instead of the initial one. |
| |
| Keywords: | Agro-antibiotic 2-16 Mutation SAS software Medium optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
