首页 | 本学科首页   官方微博 | 高级检索  
   检索      

传统分离培养结合DGGE法检测榨菜腌制过程的细菌多样性
引用本文:李正国,付晓红,邓 伟,杨迎伍,崔英双,赵 平.传统分离培养结合DGGE法检测榨菜腌制过程的细菌多样性[J].微生物学通报,2009,36(3):0371-0376.
作者姓名:李正国  付晓红  邓 伟  杨迎伍  崔英双  赵 平
作者单位:1. 重庆大学生物工程学院基因工程研究中心,重庆市高校功能基因与调控新技术重点实验室,重庆,400044
2. 重庆涪陵榨菜(集团)有限公司,重庆,408000
基金项目:重庆市科委自然基金重点项目(No. CSTC 2007BA1005)
摘    要:采用传统分离培养和基于16S rRNA 作为分子标记的变性梯度凝胶电泳(Denaturing gradient gel electrophoresis, DGGE)的方法, 分析榨菜腌制过程中不同时期的可培养细菌数量、多样性及其群落结构。结果表明, 用传统分离与分子鉴定方法获得7个属的细菌类群, 其中乳杆菌属(Acidobacterium)是优势菌群, 明串珠菌属(Leuconostoc)是次优势菌群。对通过DGGE方法得到的11条16S rRNA优势条带序列进行了比对, 结果表明明串珠菌属(Leucon

关 键 词:榨菜(Brassica  juncea  coss  var.  tsatsai)    16S  rRNA    传统分离培养    变性梯度凝胶电泳(DGGE)    群落多样性

Analysis of Bacterial Diversity During the Processing of Brassica juncea coss var. tsatsai by the Culture-independent and Denaturing Gradient Gel Electrophoresis (DGGE) Methods
LI Zheng-Guo,FU Xiao-Hong,DENG Wei,YANG Ying-Wu,CUI Ying-Shuang and ZHAO Ping.Analysis of Bacterial Diversity During the Processing of Brassica juncea coss var. tsatsai by the Culture-independent and Denaturing Gradient Gel Electrophoresis (DGGE) Methods[J].Microbiology,2009,36(3):0371-0376.
Authors:LI Zheng-Guo  FU Xiao-Hong  DENG Wei  YANG Ying-Wu  CUI Ying-Shuang and ZHAO Ping
Institution:Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China;Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China;Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China;Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China;Chongqing Fuling Zhacai Group Ltd., Chongqing 408000, China;Chongqing Fuling Zhacai Group Ltd., Chongqing 408000, China
Abstract:Both culture-dependent and culture-independent methods, denaturing gradient gel electrophoresis (DGGE) based on the sequence of 16S rRNA V3 region gene, were used to examine the total bacteria counts, bacterial diversity and community structure of different periods Brassica juncea saline water samples. The results showed that 7 genera of bacteria were identified from 19 isolated bacterial populations by the traditional isolation method, the dominant bacteria in intestine belonged to Acidobacterium. By 16S rRNA V3 region gene DGGE method, 11 distinct bands were obtained from 16S rDNA amplifications. The profile of DGGE fingerprints of different periods saline water revealed that the most dominant bacteria group was Leuconostoc in the process of the fermentation. This suggested that a combination of molecular and traditional culture methods can be used to analyze and monitor the diversity of the process of fermentation microflora effectively, and that will give us more information of microorganism diversity.
Keywords:Brassica juncea var  tsatsai  Microbial diversity  Traditionaly isolation culture  Denaturing gradient gel electrophoresis (DGGE)  16S rRNA
本文献已被 万方数据 等数据库收录!
点击此处可从《微生物学通报》浏览原始摘要信息
点击此处可从《微生物学通报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号