杆状病毒介导的基因传递逃避补体攻击的措施

血清补体系统是杆状病毒介导基因传递过程中的主要抑制因素.概述逃避补体攻击的有效措施:选择免疫赦免组织作为杆状病毒介导基因传递的靶标,例如眼睛、脑和睾丸；体外转导也能有效地逃避补体的攻击；利用补体的药理抑制剂或者对杆状病毒载体进行加工修饰,例如聚合物包被、假型化和展示补体抑制剂等,可以消除杆状病毒对补体的敏感性.有效地逃避补体攻击有助于杆状病毒广泛应用于基因治疗.

The measures of avoiding complement attack in Baculovirus-mediated gene delivery

Serum complement system is the signiflcant barrier to the development of bacu-lovirus vectors for therapeutic gene delivery.This paper gave a review to the efforts taken to avoid complement attack.Selecting the immunoprivileged tissues as target for baculovi-rus-mediated gene delivery,such as eye,brain and testis.ex vivo transduction is a widely used approach to exclude the complement attack.Using of pharmacological inhibitors of comple-ment as well as surface engineering of the baculoviral vectors through the use of synthetic polymers,pseudotyping or display of complement inhibitors can inhibit complement attack ef-ficiently.This will signiflcantly increase the possibility of using baculovirus vectors for thera-peutic applications.