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酸马奶中乳酸菌的鉴定及生物学特性的研究
引用本文:霍小琰,李少英,郭荣荣.酸马奶中乳酸菌的鉴定及生物学特性的研究[J].微生物学通报,2012,39(7):0940-0948.
作者姓名:霍小琰  李少英  郭荣荣
作者单位:内蒙古农业大学食品科学与工程学院 内蒙古呼和浩特010018
基金项目:内蒙古自治区高等学校科学研究项目(No. NJ09059); 国家自然科学基金项目(No. 31060014)
摘    要:目的]对从酸马奶中分离出来的10株乳酸菌进行鉴定和生理生化特性研究,为工业生产筛选特性优良的菌种.方法]通过形态学观察、生理生化特性、分子生物学特性及其对致病菌抑制作用的研究对其进行鉴定,并筛选特性优良菌株.结果]10株乳酸菌分别为2株Lactobacillus plantarum、2株Enterococcus villorum、2株Enterococcus dispar、3株Enterococcus durans和1株Enterococcus raffinosus;其对Staphylococcus aureus、Escherichia coli和Enteritidis bacillus有不同程度的抑制作用.结论]菌株HZ24、HZ25具有良好的生物学特性和益生功能,可以应用到食品发酵工业生产中.

关 键 词:酸马奶  乳酸菌  鉴定  生物学特性

Identification and biological characteristics of lactic acid bacteria isolated from koumiss
HUO Xiao-Yan,LI Shao-Ying and GUO Rong-Rong.Identification and biological characteristics of lactic acid bacteria isolated from koumiss[J].Microbiology,2012,39(7):0940-0948.
Authors:HUO Xiao-Yan  LI Shao-Ying and GUO Rong-Rong
Institution:College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010018, China
Abstract:Objective] The aim of the present study was to identify the genera and screen potential characteristics bacteria in the lactic acid bacteria isolated from koumiss. Methods] The characteristics of lactic acid bacteria were studied by the experiments on some aspects, such as morphology, physiological and biochemical characteristics, molecular biology and the inhibition of pathogenic bacteria. Results] There were two strains of Lactobacillus plantarum, two strains of Enterococcus villorum, two strains of Enterococcus dispar, three strains of Enterococcus durans and one strain of Enterococcus raffinosus. The tested bacteria had different degrees of inhibition to Staphylococcus aureus, Escherichia coli and Enteritidis bacillus. Conclusion] HZ24 and HZ25 were good potential probiotics which may make great contributions to food fermentation industry.
Keywords:Koumiss  Lactic acid bacteria  Identification  Biological characteristics
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