| 一株产木聚糖酶链霉菌的鉴定及发酵产酶* |
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| 引用本文： | 李里特, 丁长河, 江正强, 石波,.一株产木聚糖酶链霉菌的鉴定及发酵产酶*[J].微生物学通报,2003,30(6):59-64. |
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| 作者姓名： | 李里特 丁长河 江正强 石波 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业科学院饲料研究所,北京,100081 |
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| 基金项目： | 国家“十五”科技攻关计划项目(No.2001BA708B04-06) |
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| 摘 要： | 以木聚糖为唯一碳源，筛选出一株高产木聚糖酶生产菌株。该菌株经形态特征、 培养特征、生理生化和细胞壁组分分析等实验，鉴定为卷须链霉菌（Streptomyces cirratus).摇瓶发酵产酶实验表明：培养基最佳初始pH值为6．0；玉米芯水不溶木聚糖和蛋白胨分别是最佳的碳源和氮源；添加0．5％吐温80使得木聚糖酶活力提高到原来的2．5倍，发酵液最高酶活达到623u／mL。
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| 关 键 词： | 链霉菌 鉴定 木聚糖酶 发酵条件 |
| 文章编号： | 0253-2654(2003)06-0059-06 |
| 修稿时间： | 2003年1月20日 |
IDENTIFICATION OF A XYLANASE PRODUCING STRAIN OF STREPTOMYCES SP. AND OPTIMIZATION OF CONDITIONS ON ITS ENZYME PRODUCTION |
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| LI Li-Te DING Chang-He JIANG Zheng-Qiang SHI Bo.IDENTIFICATION OF A XYLANASE PRODUCING STRAIN OF STREPTOMYCES SP. AND OPTIMIZATION OF CONDITIONS ON ITS ENZYME PRODUCTION[J].Microbiology,2003,30(6):59-64. |
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| Authors: | LI Li-Te DING Chang-He JIANG Zheng-Qiang SHI Bo |
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| Abstract: | A xylanase producing strain was screened with xylan as the only carbon source. The strain was identified as Streptomyces cirratus. The effects of different factore on the enzyme production were studied. Corncobs xylan (water insoluble) and tryptone were the best C and N sources, respectively. The enzyme activity was increased to about 2.5 times by addition of 0.5% Tween 80 in the medium. The highest xylanase activity was up to 623u/mL. |
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| Keywords: | Streptomyces cirratus Identification Xylanase Production |
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