| 辐射污染区土壤中放线菌的分离及多样性 |
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| 引用本文： | 张志东,茆 军,唐琦勇,王 玮,谢玉清,石玉瑚.辐射污染区土壤中放线菌的分离及多样性[J].微生物学通报,2009,36(9):1329-1333. |
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| 作者姓名： | 张志东 茆 军 唐琦勇 王 玮 谢玉清 石玉瑚 |
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| 作者单位： | 新疆农业科学院微生物研究所,新疆,乌鲁木齐,830091 |
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| 基金项目： | 农业部农业公益性行业科研专项经费项目资助(No. 200803034); 国家863计划项目(No. 2007AA021300) |
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| 摘 要： | 从辐射污染区采集42份土样, 分别采用6种分离培养基进行放线菌的分离, 共获得152株放线菌。经形态、核糖体DNA扩增片段限制性内切酶(Amplifed ribosomal DNA restriction analysis, ARDRA)分析比较, 选取其中的60株进行16S rRNA基因测序。通过序列比对、聚类分析, 60株菌分布在放线菌纲中的12个属, 链霉菌属占大多数, 有大量稀有放线菌, 这表明辐射污染区具有较丰富的放线菌属种多样性。
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| 关 键 词： | 辐射污染土壤 放线菌 多样性 |
Diversity Investigation of Actinomycetes Isolated from Radiation-polluted Soil |
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| ZHANG Zhi-Dong,MAO Jun,TANG Qi-Yong,WANG Wei,XIE Yu-Qing and SHI Yu-Hu.Diversity Investigation of Actinomycetes Isolated from Radiation-polluted Soil[J].Microbiology,2009,36(9):1329-1333. |
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| Authors: | ZHANG Zhi-Dong MAO Jun TANG Qi-Yong WANG Wei XIE Yu-Qing and SHI Yu-Hu |
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| Institution: | Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China;Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China;Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China;Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China;Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China;Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urmqi, Xinjiang 830091, China |
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| Abstract: | One hundred and fifty two actinomycetes were isolated from forty two radiation-polluted soil samples, using six different isolation media. Sixty cultures were chosen for 16S rRNA gene sequence and systematic analysis, which based on their morphology and ARDRA. Results of 16S rRNA gene sequences blasting showed that the strains were assigned to 12 recognized genera of actinomycetes, most of them fall within Streptomyces genus and a great deal of strains belonged to rare actinomycetes, which indicated a rich diversity of actinomycetes in the radiation-polluted soil. |
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| Keywords: | Radiation-polluted soil Actinomycete Diversity |
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