酵母菌HL的批次发酵及发酵动力学初探

在摇瓶培养的基础上，对酵母菌Ｌｉｐｏｍｙｃｅｓ ｓｔａｒｋｅｙｉ ＨＬ进行了小型发酵罐的分批和分批补料发酵及其发酵动力学的初步研究。结果表明，通过后期补料既可明显地延长菌体脂类合成期，减缓油脂比合成速率的降低，又可增加菌液的细胞密度，最终提高了整个发酵罐的油脂产量和平均容量产率。发酵结果如下：发酵时间１２０ｈ；油脂产量１１.０ｇ／Ｌ；菌体生物量１９．４ｇ／Ｌ。油脂百分含量 ５６．５％，显然比分批培养８４ｈ所得的１１．２ｇ／Ｌ细胞生物量和 ６.１ｇ／Ｌ油脂产量分别增长了７３％和８０％。此外，通

STUDIES ON THE BATCH-FERMENTENT AND THE FERMENTENT KINETICS OF YEAST-L STARKEYI HL

Based on the flask culture conditions, the batch and fed batch of fermentor cultures were performed in a small fermentor. The kinetics of growth and lipid producing were also studied, and the result indicated that using a fed-batch couldn't only prolong the period of lipid synthesis but also markedly retard the fast decreasing of specific lipid productivity after the time of sugar exhaustion. besides these, the cell density and the amount of lipid could also be increased. A content of 11.og/L lipid and 19.4g/L dry biomass were determined at 120h and the cell contained 56.5% lipid by using a fed-batch at the later period of fermentaion.