| 红树林植物促生菌SZ7-1 菌株的培养基优化 |
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| 引用本文： | 袁辉林,康丽华,王胜坤.红树林植物促生菌SZ7-1 菌株的培养基优化[J].微生物学通报,2011,38(3):333-340. |
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| 作者姓名： | 袁辉林 康丽华 王胜坤 |
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| 作者单位： | 中国林业科学研究院热带林业研究所,广东,广州,510520 |
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| 基金项目： | 广东省科技创新课题(No. 2009KJCX004-03); 国家级林业成果推广项目(No. [2010]10 号); 国家级星火计划项目(No. 2005EA169010); 林业公益性行业科研专项(No. 201004075) |
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| 摘 要： | 采用响应面分析法对红树林植物促生菌SZ7-1 菌株发酵培养基进行了优化。首先利用Plackett-Burman (PB)设计对影响SZ7-1 生长的11 个营养因素进行评价, 并筛选出显著影响因子:玉米糖浆、酵母膏和K2HPO4; 其次用最陡爬坡实验逼近以上三因素最优水平; 最后采用响应面法对该3 个显著因素的最佳水平范围进行研究, 得到的最佳浓度为: 玉米浆28 g/L、酵母膏14 g/L和K2HPO4 2.2 g/L。在此条件下, 培养20 h 后SZ7-1 菌数达到2.6×1010 CFU/mL, 与优化前基础培养基、LB 和牛肉膏蛋白胨培养基中生长的菌数相比, 分别提高了12.4、2.4 和5.5 倍。
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| 关 键 词： | SZ7-1 Plackett-Burman 设计 Box-Behnken 设计 Minitab 软件 响应面法 |
| 收稿时间： | 9/7/2010 12:00:00 AM |
| 修稿时间： | 2010/12/7 0:00:00 |
Optimization of growth medium for a PGPR strain (SZ7-1) from mangrove rhizosphere |
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| YUAN Hui-Lin,KANG Li-Hua and WANG Sheng-Kun.Optimization of growth medium for a PGPR strain (SZ7-1) from mangrove rhizosphere[J].Microbiology,2011,38(3):333-340. |
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| Authors: | YUAN Hui-Lin KANG Li-Hua and WANG Sheng-Kun |
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| Institution: | Research Institute of Tropical Forestry, CAF, Guangzhou, Guangdong 510520, China;Research Institute of Tropical Forestry, CAF, Guangzhou, Guangdong 510520, China;Research Institute of Tropical Forestry, CAF, Guangzhou, Guangdong 510520, China |
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| Abstract: | A plant growth-promoting rhizobacteria(PGPR) strain,SZ7-1,was obtained from mangrove rhizosphere and tested previously for potential applications.To develop an economical and practical fermentation medium for the growth of SZ7-1,response surface method was used in this study.Plack-ett-Burman factorial design was used to examine the significance of 11 different media on the growth of SZ7-1.Experimental result demonstrated that media consisting of corn syrup,yeast extract and K2HPO4 significantly improved the... |
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| Keywords: | SZ7-1 Plackett-Burman design Box-Behnken design Minitab software Response surface methodology |
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