| 三唑磷降解菌株GS-1的分离鉴定及其降解特性的研究 |
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| 引用本文： | 郭新强,李 荣,林栋青,朱 彬,李顺鹏,蒋建东.三唑磷降解菌株GS-1的分离鉴定及其降解特性的研究[J].微生物学通报,2009,36(8):1143-1149. |
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| 作者姓名： | 郭新强 李 荣 林栋青 朱 彬 李顺鹏 蒋建东 |
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| 作者单位： | 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金(No. 30600016); 国家高技术研究发展计划863计划(No. 2007AA10Z405); 国家科技基础平台建设项(No. 2005DKA21201-2); 江苏省科技攻关项目(No. BE2008669); 广东省教育部产学研结合项目(No.2006090204007) |
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| 摘 要： | 从有机磷农药污水处理池污泥中分离到一株能高效降解三唑磷的菌株GS-1, 通过生理生化实验和16S rDNA序列同源性分析, 将该菌株鉴定为Diaphorobacter sp.。菌株GS-1能以三唑磷为唯一碳源生长, 能在12 h内降解100 mg/L的三唑磷至检测不出的水平。菌株GS-1降解三唑磷的过程中会产生中间代谢产物苯唑醇(1-苯基-3-羟基-1,2,4-三唑), 36 h后苯唑醇被完全转化。菌株GS-1降解三唑磷的最适pH值为8.0, 最适温度为30°C, 且对杀螟硫磷、辛硫磷、毒死蜱和甲基对硫磷
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| 关 键 词： | 三唑磷 降解 Diaphorobacter sp. 趋化性 苯唑醇 |
Isolation and Characterization of a Triazophos-degrading Strain GS-1 and Its Degrading Characteristics |
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| GUO Xin-Qiang,LI Rong,LIN Dong-Qing,ZHU Bin,LI Shun-Peng and JIANG Jian-Dong.Isolation and Characterization of a Triazophos-degrading Strain GS-1 and Its Degrading Characteristics[J].Microbiology,2009,36(8):1143-1149. |
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| Authors: | GUO Xin-Qiang LI Rong LIN Dong-Qing ZHU Bin LI Shun-Peng and JIANG Jian-Dong |
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| Institution: | Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Taizhou Entry-exit Inspection and Quarantine Bureau, Taizhou, Jiangsu 225300, China;Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China |
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| Abstract: | A strain designated as GS-1, capable of degrading triazophos efficiently, was isolated from the sludge in an organophosphate-pesticides wastewater treatment plant. Strain GS-1 was identified preliminarily as Diaphorobacter sp. based on its physiological and biochemical characters and the result of the 16S rDNA homologue sequence analysis. Strain GS-1 could grow with triazophos as its sole carbon source, and degrade 100 mg/L triazophos to non-detectable level within 12 h. The metabolite, 1-phenyl-3-hydroxy-1, 2,4-triazole, produced during triazophos biodegradation was finally transformed within 36 h. The optimal initial pH value and temperature for triazophos degradation by GS-1 was 7.0 and 30°C, respectively. Strain GS-1 could also degrade some other organophosphorus-pesticides such as fenitrothion, phoxim, chlorpyrifos and methyl-parathion. Strain GS-1 also showed chemoatxis to triazophos, fenitrothion and phoxim, indicating the close relationship between degrading and chemotaxis characteristics. This study demonstrated that strain GS-1 had great potential in the bioremediation of organophosphorus-pesticides contaminated sites. |
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| Keywords: | Triazophos Degradation Diaphorobacter sp Chemotaxis 1-phenyl-3-hydroxy-1 2 4-triazole |
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