一株细菌儿茶酚型铁载体分泌的影响因素研究

采用两种新的高分辨率的薄层层析（TLC）方法对一株土壤细菌S1在3种不同培养基上产生的儿茶酚型铁载体进行了分析。结果表明：不同培养基对铁载体的产生影响较大，在3种不同的培养基上菌株S1产生不同的儿茶酚铁载体，其中仅在1种培养基上S1能够分泌2，3-二羟基苯甲酸（2，3-DHBA）。同时，还分析了Al^3＋对S1分泌的儿茶酚型铁载体总量的影响，结果表明：Al^3＋能显著刺激铁载体的分泌，并且能抵消一定浓度范围内的Fe^2＋对铁载体分泌的抑制作用，KMB培养液中产生的4种儿茶酚铁载体中有3种和Al^3＋有较强的螯合力．

Study on Effect Factor of the Production of Catechol-type Siderophores Secreted by a Strain of Soil Bacteria

Catechol-type siderophores secreted by a strain of soil bacteria in three different medium were assayed by two high-resolution TLC.The results showed different medium had a significant effect on the secretion of catechol-type siderophores,and in three different medium strain S1 produced different catechol-type siderophores.The effect of Al~(3+) on Catechol-type siderophores by S1 were also assayed.The results showed Al~(3+)had a significant stimulation on the secretion of catechol-type siderophores.Moreover,Al~(3+)could to some extent counteract the repression of Fe~(2+)on siderophores production.In KMB medium four catechol-type siderophores were identified and all ones except for 2,3-dihydroxybenzoic acid(2,3-DHBA) had high affinity for Al~(3+).