| 三硝酸丙三酯对厌氧真菌和甲烷菌代谢产物、纤维水解酶活性及菌群丰度的影响 |
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| 引用本文： | 孟振祥,李袁飞,孙若琳,金巍,成艳芬,朱伟云.三硝酸丙三酯对厌氧真菌和甲烷菌代谢产物、纤维水解酶活性及菌群丰度的影响[J].微生物学通报,2018,45(6):1295-1302. |
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| 作者姓名： | 孟振祥 李袁飞 孙若琳 金巍 成艳芬 朱伟云 |
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| 作者单位： | 国家动物消化道营养国际联合研究中心江苏省消化道营养与动物健康重点实验室南京农业大学消化道微生物研究室 |
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| 基金项目： | 国家重点研发计划(2017YFD0500505)；国家自然科学基金(31301999) |
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| 摘 要： | 【背景】近年来硝酸酯化合物抑制瘤胃甲烷生成的能力受到广泛关注,但鲜有研究关注其对瘤胃内主要纤维降解菌——厌氧真菌的影响。【目的】研究三硝酸丙三酯(Nitroglycerin,NG)对厌氧真菌和甲烷菌活性和代谢的影响。【方法】利用厌氧真菌(Piromyces sp.F1)纯培养及与甲烷菌共培养(Piromyces sp.F1+Methanobrevibacter sp.),比较不同剂量NG(0.0、6.6、13.2和19.8μmol/L)对厌氧真菌和甲烷菌的代谢终产物、主要纤维水解酶活性及菌群丰度的影响。【结果】厌氧真菌共培养组中,6.6μmol/L NG完全抑制了甲烷的生成,积累了氢气和甲酸(P0.05),降低了乙酸浓度以及木聚糖酶和羧甲基纤维素酶活性(P0.05),但未显著影响厌氧真菌和甲烷菌的丰度(P0.05)。厌氧真菌纯培养组中,6.6μmol/L NG显著降低了甲酸、乙酸、乙醇、木聚糖酶和羧甲基纤维素酶活性(P0.05),但总产气量和氢气产量没有显著差异(P0.05)。在2个培养体系中,随着剂量的升高,NG对厌氧真菌和甲烷菌的抑制增强。【结论】NG能够抑制甲烷菌的活性,但对厌氧真菌也有抑制作用。
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| 关 键 词： | 厌氧真菌，甲烷菌，共培养，甲烷，三硝酸丙酸酯 |
Effects of nitroglycerin on the metabolic profile, fibrolytic enzyme activities and microbial abundance of anaerobic fungi and methanogens |
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| MENG Zhen-Xiang,LI Yuan-Fei,SUN Ruo-Lin,JIN Wei,CHENG Yan-Fen and ZHU Wei-Yun.Effects of nitroglycerin on the metabolic profile, fibrolytic enzyme activities and microbial abundance of anaerobic fungi and methanogens[J].Microbiology,2018,45(6):1295-1302. |
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| Authors: | MENG Zhen-Xiang LI Yuan-Fei SUN Ruo-Lin JIN Wei CHENG Yan-Fen and ZHU Wei-Yun |
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| Institution: | Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China,Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China,Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China,Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China,Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China and Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China |
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| Abstract: | |
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| Keywords: | Anaerobic fungi Methanogens Co-culture Methane Nitroglycerin |
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