| 食品中产气荚膜梭菌LAMP快速检测方法的建立 |
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| 引用本文： | 姜侃,张东雷,陈小珍,金燕飞.食品中产气荚膜梭菌LAMP快速检测方法的建立[J].微生物学通报,2011,38(8):1288-1294. |
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| 作者姓名： | 姜侃 张东雷 陈小珍 金燕飞 |
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| 作者单位： | 浙江省质量技术监督检测研究院,浙江,杭州,310013 |
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| 基金项目： | 浙江省科技厅优先主题重点社会发展项目(No. 2009C13013); 浙江省质量技术监督系统科研项目(No. 20090209) |
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| 摘 要： | 应用环介导等温扩增技术(Loop-mediated isothermal amplification, LAMP), 针对产气荚膜梭菌特有的α毒素(CPa)基因序列分析设计特异性引物, 建立食品中产气荚膜梭菌特异性快速检测方法。研究结果表明, 所设计的引物具有良好的特异性, 5株产气荚膜梭菌均能扩增出特异性片段, 而13株非产气荚膜梭菌均未扩增出相应片段, 无假阴性或假阳性情况出现。同时, 该方法可在1 h内完成反应, 且检测灵敏度达到10 fg/μL。该方法为产气荚膜梭菌的快速检测提供了一种重要的技术手段。
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| 关 键 词： | 产气荚膜梭菌 LAMP 快速检测 食品 |
The establishment of LAMP rapid detection method for Clostridium perfringens in food |
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| JIANG Kan,ZHANG Dong-Lei,CHEN Xiao-Zhen and JIN Yan-Fei.The establishment of LAMP rapid detection method for Clostridium perfringens in food[J].Microbiology,2011,38(8):1288-1294. |
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| Authors: | JIANG Kan ZHANG Dong-Lei CHEN Xiao-Zhen and JIN Yan-Fei |
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| Institution: | Zhejiang Test Academy of Quality and Technical Supervision, Hangzhou, Zhejiang 310013, China) |
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| Abstract: | LAMP(Loop-mediated isothermal amplification,LAMP) rapid detection method for Clos-tridium perfringens in food was established,with the specific primers designed on the CPa gene.The results of the study indicated that the method had perfect specificity,as 5 strains of Clostridium per-fringens were able to amplify specific fragments,while 13 strains of non-Clostridium perfringens were not,and no false positive or false negative results occurred.The test could be done within 1 hour with the sensitivity low to ... |
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| Keywords: | Clostridium perfringens LAMP Rapid detection Food |
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