链霉菌HP-S-01降解高效氯氰菊酯的特性及其动力学

研究不同接菌量、温度、pH、装液量和农药初始浓度对链霉菌HP-S-01降解高效氯氰菊酯的影响。结果表明,在接菌量为0.6 g/L、28°C、pH 7.5和装液量为50 mL/250 mL三角瓶条件下培养3 d,该链霉菌对100 mg/L高效氯氰菊酯降解率达到96%以上。链霉菌HP-S-01还能明显降解高效氟氯氰菊酯、高效氯氟氰菊酯、右旋苯醚菊酯和胺菊酯等拟除虫菊酯农药,且降解过程符合一级动力学模型,降解半衰期分别为0.78、0.88、1.08和1.24 d。采用Andrews方程对链霉菌HP-S-01降解高效氯氰菊酯的过程进行拟合,其动力学参数为qmax=1.826 3 d?1,Ks=58.951 3 mg/L,Ki=359.378 2 mg/L,该链霉菌降解高效氯氰菊酯最佳的初始浓度为145.553 5 mg/L,试验数据与该动力学方程拟合较好。

Degradation characteristics and kinetics of beta-cypermethrin by Streptomyces sp. HP-S-01

The beta-cypermethrin degradation conditions including inoculum amount,temperature,pH,liquid volume,and initial concentration by Streptomyces HP-S-01 were systematically investigated.The results showed that Streptomyces HP-S-01 degraded beta-cypermethrin rapidly with a degradation rate up to 96% within 3 d,under the conditions of inoculum amount 0.6 g/L,28 °C,pH 7.5,and liquid volume 50 mL/250 mL.The strain also could effectively degrade beta-cyfluthrin,beta-cyhalothrin,d-phenothrin and tetramethrin.Furthermore,the degradation reaction followed first-order kinetics and half lives(T1/2) were 0.78,0.88,1.08 and 1.24 d,respectively.The Andrews model was used to describe the beta-cypermethrin degradation process.The degradation kinetic data collected fitted the model well.The calculated parameters of qmax,Ks and Ki for the model were 1.826 3 d?1,58.951 3 mg/L and 359.378 2 mg/L,respectively.And the optimal concentration of initial beta-cypermethrin was 145.553 5 mg/L.