抗锌细菌Sphingomonas sp. DX-T3-03分离、鉴定及性质

从江西德兴铜矿重金属污染土壤中筛选得到一株对重金属锌具有极强抗性的菌株,命名为DX-T3-03。对该菌株进行形态观察、生理生化试验,采用16S rRNA序列分析,鉴定该菌为鞘氨醇单胞菌属(Sphingomonas sp.)。研究其最佳生长条件及抗重金属特性。试验结果表明:该菌株的最适应生长条件为温度35°C,pH约6.7,转速150r/min;对重金属锌有极高抗性,可以达到25mmol/L及以上,并能够在多种单一及复合重金属(Cu70mg/L、Cd300mg/L、Pb400mg/L、Ni60mg/L)中生长。

Isolation, Identification and Characteristics of a Zn-resisting Bacterium, Sphingomonas sp. DX-T3-03

A strain of resistance to zinc with high concentration, named DX-T3-03, was isolated from the metal-polluted sediment of soils around Dexing copper mine. Basing on morphology, biochemical and physiological characterization, DX-T3-03 was characterized as Sphingomonas sp.. A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species. In the phylogenetic tree, the DX-T3-03 strain has the closest relative to Sphingomonas aquatilis strain JSS-7 with 99% sequence homeology. The optimum growth conditions of DX-T3-03 strain were temperature of 35°C, pH of 6.7, rotating rate of 150 r/min. It could resist zinc of 25 mmol/L or above and be able to grow in a variety of single and combined heavy metals (Cu 70 mg/L, Cd 300 mg/L, Pb 400 mg/L, Ni 60 mg/L).