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紫外线诱变深黄被孢霉选育花生四烯酸高产菌株
引用本文:于长青,李丽娜.紫外线诱变深黄被孢霉选育花生四烯酸高产菌株[J].微生物学通报,2009,36(6):0853-0857.
作者姓名:于长青  李丽娜
作者单位:黑龙江八一农垦大学食品学院,黑龙江,大庆,163319
基金项目:黑龙江省教育厅利心护肝保健乳制品关键技术研究项目(No. 10551226)
摘    要:为获得生长活力较强, 产花生四烯酸能力强的菌株, 以微生物油脂产量和花生四烯酸产量为评价指标, 采用2轮紫外线诱变的方法, 利用单因素试验确定紫外线照射时间, 并采用气相色谱分析花生四烯酸含量。试验结果表明: 紫外灯功率20 W, 照射距离30 cm, 照射时间80 s, 其致死率为76.4%。经过诱变及菌种筛选, 获得1株高产菌株Z80s2-109, 其总油脂含量为16 g/L, 花生四烯酸产量为2.34 g/L, 花生四烯酸产量比原始对照菌株提高377%, 并且遗传性能稳定。

关 键 词:花生四烯酸    深黄被孢霉    紫外线    诱变

Breeding of Arachidonic Acid Producting Strain with Mortierella Sabellina by Ultraviolet Mutation
YU Chang-Qing and LI Li-Na.Breeding of Arachidonic Acid Producting Strain with Mortierella Sabellina by Ultraviolet Mutation[J].Microbiology,2009,36(6):0853-0857.
Authors:YU Chang-Qing and LI Li-Na
Institution:Foodstuff College, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319, China;Foodstuff College, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319, China
Abstract:In order to gain the strain which has high growth vigor and whose biomass concentration satisfy further fermentation to produce arachidonic acid, we took the yield of microbes olein and arachidonic acid as evaluative index, adopted twice ultraviolet mutation, determined the time of ultraviolet irradiation by single factor expriment, and then the content of the arachidonic acid was measured by GC. The results of this experiment showed that the power of viltalight lamp was 20 W, exposure distance was 30 cm and exposure time was 80 s, lethality of spores of Mortierella isabellina was 76.4%. After twice ultraviolet mutation and repeatedly screen, a mutation of Mortierella isabellina Z80s2-109 whose arachidonic acid concentration in biomass were 3.77 times of the control strains was obtained and genetic stability.
Keywords:Arachidonic acid  Mortierella isabellina  Ultraviolet  Mutation
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