| 真菌产纤维素酶培养基中刚果红转移机理研究 |
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| 引用本文： | 张超,李艳宾,张磊,张琴,魏世清.真菌产纤维素酶培养基中刚果红转移机理研究[J].微生物学通报,2006,33(6):12-16. |
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| 作者姓名： | 张超 李艳宾 张磊 张琴 魏世清 |
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| 作者单位： | 1. 重庆市农业科学院果树研究所,重庆,402260
2. 西南大学资源与环境学院,重庆,400716 |
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| 基金项目： | 重庆市高等学校优秀青年骨干教师资助计划专项课题资助 |
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| 摘 要： | 通过对产纤维素酶真菌在纤维素刚果红液体培养基中刚果红染料移动情况研究，表明刚果红染料进入真菌的机制为纤维素分解真菌首先分解纤维素物质为含有葡聚糖等结构的多聚糖类物质，多聚糖与刚果红形成多聚糖-刚果红复合物，复合物不仅破吸附到产纤维素酶活的菌丝外表面，而且能被进一步转运吸收至该部分菌丝内部，使菌丝体和菌落呈现红色。所以，纤维素刚果红培养基可作为分离、筛选纤维素分解直菌的特异性培养基。
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| 关 键 词： | 刚果红 纤维素酶 真菌 多聚糖-刚果红复合物 机理 |
| 文章编号： | 0253-2654（2006）06-0012-05 |
| 收稿时间： | 2006-01-13 |
| 修稿时间： | 2006-03-02 |
Study on Transition Mechanism of Congo-red in Cellulase-producing Fungi Medimn |
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| ZHANG Chao,LI Yan-Bin,ZHANG Lei,ZHANG Qin,WEI Shi-Qing.Study on Transition Mechanism of Congo-red in Cellulase-producing Fungi Medimn[J].Microbiology,2006,33(6):12-16. |
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| Authors: | ZHANG Chao LI Yan-Bin ZHANG Lei ZHANG Qin WEI Shi-Qing |
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| Institution: | Fruit Research Institute, Chongqing Academy of Agricaltrual, Chongqing 402260; College of Resources and Environment, Southwest University, Chongqing 400716 |
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| Abstract: | Transition of congo-red in cellulose congo-red liquid medium was studied by cellulase-producing fungi. Results showed that cellulose was decomposed and turned into polysaccharides by cellulase-producing fungi, which possessed of glucans structure, then polysaccharides bonded congo-red to form polysaccharides-congo red complex. The complex was not only adhered on the surface of hyphae, but also absorbed inside them. With that, congo-red deposited in hyphae, and colored the colony red. So, cong-red medium can be used as a special medium to identify fungi possessing cellulase. |
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| Keywords: | Congo-red Cellulase Fungi Polysaccharides-congo red complex Mechanism |
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