烟草几丁酶β—1.3—葡聚糖酶的抑菌作用

从三个方面对激发子诱导烟草几丁质酶，β－１．３－葡聚糖酶的病理功能进行了测定，结果表明：①两种酶对赤星菌的菌落生长有抑制作用并可抑制孢子萌发，对其它被测病原物抑制作用非常微弱；②两种酶对赤星菌等有明显的攻击作用，直接攻击抱子的反应在３０ｍｉｎ内可见，到１６ｈ前后引起孢子破裂；③扫描电镜观察，酶对寄主生活叶面上的赤星菌有同样攻击作用。

INHIBITION OF TOBACCO CHITINASE AND 1.3-GLUCANASE TO SOMEPATHOGENIC FUNGI

The in vitro activity of the enzymes was observed by 3 methods. In plate inhibitory tests, can showedfeeble inhibition to the fungal growth, but neither Cht nor Clu Cht did. In microscopy, cotton blue stainingobservation Proved that the earlier reactions of the enzymes to attack the fungal spores occurred within 30 min .and direct observation without staining demonstrated the sequent events followed, including the sporal pantalplasmolysis in fly cytoplastic swelling-out in 4~6h, intracellular component leakage in 6 ~8h, cell wall break in8~12h, and complete collapse in 16~ 24h. The third method, scanning electronmicroscopy, suggested thesimilar events and process of the enzymes to attack the fungi artificially inoculated onto tobacco leaves. In all ofthe tests, Glu was more active than Cht, and Glu Cht combination expressed no coordinated effect. TobaccoGlu also attaCked pathogens of Northern and Southern corn leaf blight and wheat powdery mildew tested.