假单胞菌合成环脂肽转录调控的研究进展

假单胞菌所合成的环脂肽是一类由环状的寡肽连接一个脂肪酸链组成的两亲性分子，利用巯基化模块由非核糖体肽合成酶合成。环脂肽的生物合成受到严格、复杂的调控，GacS/GacA双组分系统和群体感应系统是其中两类重要的调控系统。本文总结假单胞菌合成环脂肽的调控机制及相关调控因子；对基于PCR的高通量分子筛选方法获取特定环脂肽进行分析，同时对基于调控机制的遗传改造提高假单胞菌产环脂肽的能力和获取更多新型环脂肽等方面的应用进行 展望。

Biosynthetic regulation of cyclic lipopeptides produced by Pseudomonas

The cyclic lipopeptides (CLPs) produced by Pseudomonas were made up of a cyclized oligopeptide lactone ring coupled to a fatty acid tail. CLPs were synthesized by nonribosomal peptide synthetases in Pseudomonas and the genetic regulation of CLPs syntheses was strict and comprehensive. GacA/GacS two-component signal transduction system and N-AHL-mediated quorum sensing system played an important role in global regulation of CLPs production in Pseudomonas. Here, we reviewed some research advances on the systems involved in CLPs syntheses. We discussed the method using PCR to obtain some cyclic lipopeptides and prospected the applications of bioengineering technology in enhancing the production of CLPs in Pseudomonas. It was also discussed the genome mining combining with genetic regulation research to obtain new cyclic lipopeptides.