| 玫瑰红链霉菌336质粒（pSR336）DNA的分离及特性研究 |
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| 引用本文： | 还连栋, 董可宁, 谭华荣, 庄增辉, 薛禹谷,.玫瑰红链霉菌336质粒（pSR336）DNA的分离及特性研究[J].微生物学通报,1996,23(3):155-160. |
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| 作者姓名： | 还连栋 董可宁 谭华荣 庄增辉 薛禹谷 |
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| 作者单位： | 中国科学院微生物研究所; |
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| 摘 要： | 从葡萄糖异构酶产生菌──玫瑰红链霉菌３３６中分离得到质粒ｐＳＲ３３６。经电泳检测和电镜观察证实，存在共价闭合超螺旋和开环两种分子构型，分子量约为６．３５ｋｂ，拷贝数约为１３０。采用高温（４０℃），吖啶橙、溴化乙锭和ＳＤＳ等物理、化学因素消除ｐＳＲ３３６，均未获得质粒消除株，表明ｐＳＲ３３６质粒是非常稳定的。用变铅青链霉菌ＴＫ２１作受体，进行平皿杂交以检测ｐＳＲ３３６的接合转移能力，未观察到麻点（ｐｏｃｋ）的形成。用ＨｉｎｄⅢ分别酶切ｐＳＲ３３６和ｐＩＪ４８６，连接得到一个嵌合质粒ｐＩＲ３０，检测玫瑰红链霉
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| 关 键 词： | 玫瑰红链霉菌 质粒 |
ISOLATION AND CHARACTERIZATION OF PLASMID pSR336 FROM GLUCOSE ISOMERASE PRODUCING STRAIN STREPTOMYCES ROSEORUBER 336 |
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| Huan Liandong, Dong Kening, Tan Huarong, Zhuang Zenghui, Xue Yugu.ISOLATION AND CHARACTERIZATION OF PLASMID pSR336 FROM GLUCOSE ISOMERASE PRODUCING STRAIN STREPTOMYCES ROSEORUBER 336[J].Microbiology,1996,23(3):155-160. |
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| Authors: | Huan Liandong Dong Kening Tan Huarong Zhuang Zenghui Xue Yugu |
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| Abstract: | Plasmid DNA has been isolated from glucose isomerase producing strainStreptomyces roseoruber 336. The plasmid was designated PSR336. Electron microscopic photographs demonstrated the existence of cccDNA and ocDNA. The molecularweight of PSR336 was about 6.35kb. The experimental results indicated that no relationship between plasmid DNA and glucose isomerase production was found. |
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| Keywords: | Streptomyces Plasmid |
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