| 长双歧杆菌BBMN68 可溶性蛋白质图谱的建立 |
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| 引用本文： | 肖满,徐攀,赵建云,马会勤,张江巍,陈尚武.长双歧杆菌BBMN68 可溶性蛋白质图谱的建立[J].微生物学通报,2011,38(3):417-423. |
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| 作者姓名： | 肖满 徐攀 赵建云 马会勤 张江巍 陈尚武 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,教育部北京市功能乳品实验室,北京,100083
2. 中国农业大学农学与生物技术学院,北京,100193 |
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| 基金项目： | 国家自然科学基金项目(No. 31071507) |
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| 摘 要： | 为了建立长双歧杆菌BBMN68蛋白质图谱,采用双向电泳的方法建立了2-D参考图谱,通过MALDI-TOF/MS质谱鉴定和数据库搜索,鉴定到206个蛋白质(占长双歧杆菌BBMN68基因预测总蛋白的11.4%)。通过2-D胶分析,共有800±15(对数期)和800±20(稳定期)个蛋白质,其中282个蛋白点成功鉴定,代表206个不同的蛋白质。另外,分析了实验鉴定蛋白质的等电点和分子量,蛋白功能,密码子偏好性,蛋白质疏水性以及蛋白质细胞定位的分析。研究结果为长双歧杆菌的比较蛋白质组学研究提供了参考图谱和蛋白质基础信息数据。
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| 关 键 词： | 长双歧杆菌BBMN68 蛋白质组 双向电泳 蛋白质图谱 |
| 收稿时间： | 2010/9/19 0:00:00 |
| 修稿时间： | 2010/12/3 0:00:00 |
A proteome reference map of Bifidobacterium longum subsp. longum BBMN68 |
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| XIAO Man,XU Pan,ZHAO Jian-Yun,MA Hui-Qin,ZHANG Jiang-Wei and CHEN Shang-Wu.A proteome reference map of Bifidobacterium longum subsp. longum BBMN68[J].Microbiology,2011,38(3):417-423. |
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| Authors: | XIAO Man XU Pan ZHAO Jian-Yun MA Hui-Qin ZHANG Jiang-Wei and CHEN Shang-Wu |
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| Institution: | Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China;Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | To establish proteomic maps of Bifidobacterium longum subsp.longum BBMN68,applying two-dimensional gel electrophoresis,we built a 2-D reference map,by MALDI-TOF mass spectrometry.We identified a total number of 206 different proteins(11.4% of predicted total proteins in B.longum subsp.longum BBMN68).A total of 800±15(exponential) and 800±20(stationary) protein spots on each 2D-PAGE map were got,in which a total of 282 spots were successfully identified,represented 206 different proteins.Additionally,combined with analysis of pI and molecular masses,COG,CAI,GRVAVY and the cellular localizations of identified proteins in B.longum subsp.longum BBMN68.This study presented a proteome map of B.longum BBMN68 and provided a reference proteome data-base for comparative proteomic studied. |
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| Keywords: | Bifidobacterium longum subsp longum BBMN68 Proteomics Two-dimensional gel electrophoresis Reference map |
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