首页 | 本学科首页   官方微博 | 高级检索  
   检索      

重组巴斯德毕赤酵母发酵生产几丁质酶的条件优化研究
引用本文:阎瑞香,吴仲,侯建华,李明钢.重组巴斯德毕赤酵母发酵生产几丁质酶的条件优化研究[J].微生物学通报,2007,34(3):0468-0471.
作者姓名:阎瑞香  吴仲  侯建华  李明钢
作者单位:1. 南开大学分子生物学研究所生物活性材料教育部重点实验室,天津,300071;国家农产品保鲜工程技术研究中心,天津,300384
2. 西北农林科技大学食品学院,杨凌,712100
3. 南开大学分子生物学研究所生物活性材料教育部重点实验室,天津,300071
基金项目:天津市应用基础研究项目
摘    要:研究了利用重组巴斯德毕赤酵母诱导表达重组几丁质酶的条件。在摇瓶水平上研究了诱导时间、pH、甲醇流加量、油酸等因素对重组几丁质酶表达的影响。结果发现诱导108h蛋白表达量最高;偏酸性环境不利于蛋白表达,维持在pH5.5~6.0最佳;甲醇最佳诱导浓度为1%;添加0.05%的油酸有助于提高蛋白表达量。在此基础上通过正交试验设计优化了培养基配方,在优化条件下,蛋白表达量达171.99mg/L,酶活达49.58U/mL。

关 键 词:毕赤酵母  重组几丁质酶  发酵  优化
文章编号:0253-2654(2007)03-0468-04
修稿时间:2006-07-20

The Research on Optimal Culture Condition of Recombintant Chitinase Production in Pichia pastoris
YAN Rui-Xiang,WU Zhong,HOU Jian-Hua and LI Ming-Gang.The Research on Optimal Culture Condition of Recombintant Chitinase Production in Pichia pastoris[J].Microbiology,2007,34(3):0468-0471.
Authors:YAN Rui-Xiang  WU Zhong  HOU Jian-Hua and LI Ming-Gang
Institution:1. The Key Laboratory for Bioactivematerial of Ministry of Education, Institute for Molecular Biology, College of Life Science, Nankai University, Tianjin 300071 ;2. National Engineering and Technology Research Center of Agriculture Products Freshness Protection, Tianjin 300384; 3. College of Food Science and Engineering ,Northwest Agriculture and Forestry University, Yangling 712100
Abstract:The fermentation condition of a recombinant strain to produce chitinase in Pichia pastoris was studied in this paper. By the shaking flask, the influences of inducing time, pH, methanol, oleic acid on the expression of chitinase were investigated. Results showed that the highest productivity of chitinase was obtained when induced for 108 hoots. The expression of chitinase was repressed when pH was lower than 5.5 and the optimum condition was pH 5.5 - 6.0. The optimum concentration of methanol was 1%. The protein expression can be improved greatly when added 0.05 % oleic acid. On the basis of these, the recipe of fermentation medium was optimized through orthognnal experimental design. The optimal conditions were found and the amount of protein expression reached 171.99mg/L, chitinase activity 49.58U/mL under the conditions.
Keywords:P  pastoris  Recombinant chitinase  Fermentation  Optimization
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《微生物学通报》浏览原始摘要信息
点击此处可从《微生物学通报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号