| 基因工程FR-008/杀念菌素脱羧衍生物CS103的分离提取及毒性和抗白色念珠菌活性研究 |
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| 引用本文： | 毛相朝,陶欣艺,杨 亮,沈亚领,魏东芝,邓子新.基因工程FR-008/杀念菌素脱羧衍生物CS103的分离提取及毒性和抗白色念珠菌活性研究[J].微生物学通报,2009,36(12):1859-1864. |
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| 作者姓名： | 毛相朝 陶欣艺 杨 亮 沈亚领 魏东芝 邓子新 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237; 中国科学院青岛生物能源与过程研究所 中国科学院生物燃料重点实验室 山东 青岛 266101;华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237;华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237;华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237;华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237; 中国科学院青岛生物能源与过程研究所 中国科学院生物燃料重点实验室 山东 青岛 266101;上海交通大学微生物代谢教育部重点实验室 上海 200030 |
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| 基金项目： | 上海市创新行动计划重大项目(No. 2007DZ19503-4) |
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| 摘 要： | 本文在建立了基因工程FR-008/杀念菌素脱羧衍生物CS103分离提取工艺的基础上, 经进一步纯化, 获得一定供试样品。通过对比脱羧FR-008/杀念菌素CS103、FR-008/杀念菌素和两性霉素B三种化合物对人胚肾细胞毒性、对人血红细胞的溶血活性和对白色念珠菌的抗菌效果, 发现脱羧FR-008/杀念菌素CS103的毒性较FR-008/杀念菌素和两性霉素B大大降低, 且保持了较高的抗真菌(Candida albicans)活性。
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| 关 键 词： | FR-008/杀念菌素 脱羧衍生物CS103 两性霉素B 毒性 抗真菌活性 |
Research on the Isolation, Cell Toxicity and Antifungal Activity Against Candida albicans of Novel Decarboxylated FR-008/Candicidin Derivative CS103 |
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| MAO Xiang-Zhao,TAO Xin-Yi,YANG Liang,SHEN Ya-Ling,WEI Dong-Zhi and DENG Zi-Xin.Research on the Isolation, Cell Toxicity and Antifungal Activity Against Candida albicans of Novel Decarboxylated FR-008/Candicidin Derivative CS103[J].Microbiology,2009,36(12):1859-1864. |
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| Authors: | MAO Xiang-Zhao TAO Xin-Yi YANG Liang SHEN Ya-Ling WEI Dong-Zhi and DENG Zi-Xin |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; 2. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chine;State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;1. State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; 2. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chine;Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China |
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| Abstract: | Based on founding the methods for isolation and purification of the novel decarboxylated FR-008/Candicidin derivative CS103, we obtained enough samples for testing from the culture mycelia of the mutant of Streptomyces FR-008. Through comparing the cell toxicities on Human Embryonic Kidney Cells 293, haemolytic activities on human erythrocytes and antifungal activities on C. albicans, we found that the toxicity of decarboxylated FR-008/Candicidin derivative CS103 had been lower than FR-008/Candicidin and Amphoteicin B, while it still had high antifungal activity on C. albicans. |
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| Keywords: | FR-008/Candicidin Novel decarboxylated derivative Amphoteicin B Toxicities Antifungal activities |
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