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| 洋葱伯克霍尔德菌T1828质粒消除方法及条件 | |
| 引用本文： | 钟义军,吴晓玉,刘好桔,武琳,饶志强,王园秀.洋葱伯克霍尔德菌T1828质粒消除方法及条件[J].微生物学通报,2011,38(6):847-852. |
| 作者姓名： | 钟义军 吴晓玉 刘好桔 武琳 饶志强 王园秀 |
| 作者单位： | 1. 江西农业大学生物科学与工程学院，江西南昌330045;江西省红壤研究所，江西南昌331717;南昌市发酵应用技术重点实验室，江西南昌330045 2. 江西农业大学生物科学与工程学院，江西南昌330045;南昌市发酵应用技术重点实验室，江西南昌330045 3. 江西省红壤研究所,江西南昌,331717 |
| 基金项目： | 江西省自然科学基金项目(No. 2008GJN0016); 江西省教育厅科技项目(No. GJJ09171); 江西农业大学校基金项目(No. 2548) |
| 摘 要： | 采用十二烷基硫酸钠和提高生长温度结合法、紫外线涂布平板法、冻融法、吖啶橙法和黄芩甙提取液消除法等方法消除洋葱伯克霍尔德菌T1828菌株质粒,探讨适合洋葱伯克霍尔德菌质粒消除的方法,并研究最佳质粒消除条件。结果表明十二烷基硫酸钠和提高生长温度结合法最适合于洋葱伯克霍尔德菌T1828质粒消除,同时最佳消除条件为:在T1828培养16 h后加入SDS使其终浓度0.1%,36°C处理18 h,消除率达到49%。筛选到的无质粒突变株可以用于进一步的研究。 |
| 关 键 词： | 洋葱伯克霍尔德菌T1828 质粒消除 质粒提取 消除条件 |
Plasmid elimination method and conditions of Burkholderia cepacia T1828 |
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| ZHONG Yi-Jun,WU Xiao-Yu,LIU Hao-Ju,WU-Lin,RAO Zhi-Qiang and WANG Yuan-Xiu.Plasmid elimination method and conditions of Burkholderia cepacia T1828[J].Microbiology,2011,38(6):847-852. | |
| Authors: | ZHONG Yi-Jun WU Xiao-Yu LIU Hao-Ju WU-Lin RAO Zhi-Qiang and WANG Yuan-Xiu |
| Institution: | 1. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 2. Red Soil Institute of Jiangxi Province, Nanchang, Jiangxi 331717, China; 3. Nanchang Key Laboratory of Fermentation Application and Technology,;1. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 3. Nanchang Key Laboratory of Fermentation Application and Technology, Nanchang, Jiangxi 330045, China;1. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 3. Nanchang Key Laboratory of Fermentation Application and Technology, Nanchang, Jiangxi 330045, China;2. Red Soil Institute of Jiangxi Province, Nanchang, Jiangxi 331717, China;1. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 3. Nanchang Key Laboratory of Fermentation Application and Technology, Nanchang, Jiangxi 330045, China;1. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 3. Nanchang Key Laboratory of Fermentation Application and Technology, Nanchang, Jiangxi 330045, China |
| Abstract: | To explore the suitable for Burkholderia cepacia plasmid elimination method and study the optimal conditions for plasmid elimination, we eliminated plasmid of Burkholderia cepacia T1828 by using sodium dodecyl sulfate and improving the growth temperature combination method, UV-coated plate method, freeze-thaw method, acridine orange method and baicalin extraction methods such as elimination methods. The results showed that sodium dodecyl sulfate and improving the growth temperature combination method is best suited to Burkholderia cepacia T1828 plasmid elimination, while the best conditions for the elimination are T1828 culture after 16 h by adding SDS to 0.1% of the final solubility dealt with 18 h at 36 °C, the elimination rate reach 49%. The plasmid-free mutant strains can be used for further study. |
| Keywords: | Burkholderia cepacia T1828 plasmid elimination plasmid extraction eliminate conditions |
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