多位点测序分型技术在病原微生物分型鉴定中的应用

多位点测序分型(Multilocus sequence typing,MLST)技术是一种以核苷酸序列为基础的病原菌分型方法,它是高通量测序技术与成熟的群体遗传学相结合的产物。该方法简单易行,重复性强,可以通过国际互联网对某一致病菌株在全球范围内的传播分布情况进行追踪监控。目前,MLST技术已被广泛应用于原核病原菌及一些真核病原菌(如真菌)的分型鉴定中。主要对MLST技术的原理及其在一些常见病原菌分型鉴定中的应用进行了简要的阐述。

Application of Multilocus Sequence Typing method on Pathogenic Microorganisms Typing and Identification

It is important for identifying,tracking and preventing pathogenic microorganism's outbreak in the epidemiology of infectious diseases.Establish a useful simple method for typing and identifying pathogenic microorganisms is necessary.In 1998,Multilocus sequence typing was proposed which is a nucleotide sequence based approach.It combined developments in high-throughput sequencing techniques with established population genetics.This typing method is a portable and reproducible typing system which can track and investigate pathogenic microbes distributed condition over the global by the internet.Now,MLST schemes have been developed for a variety of prokaryotic and some eukaryotic pathogens(like some fungus).In this paper,the theory of MLST and its application on some pathogenic microbes typing and identifying were introduced.