| CRISPR/Cas9技术在微生物研究中的应用进展 |
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| 引用本文： | 张昆,陈景超,李祎,刘磊,王海磊.CRISPR/Cas9技术在微生物研究中的应用进展[J].微生物学通报,2018,45(2):451-464. |
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| 作者姓名： | 张昆 陈景超 李祎 刘磊 王海磊 |
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| 作者单位： | 河南师范大学生命科学学院 河南省功能微生物绿色转化技术工程实验室 河南 新乡 453007,河南师范大学生命科学学院 河南省功能微生物绿色转化技术工程实验室 河南 新乡 453007,河南师范大学生命科学学院 河南省功能微生物绿色转化技术工程实验室 河南 新乡 453007,河南师范大学生命科学学院 河南省功能微生物绿色转化技术工程实验室 河南 新乡 453007,河南师范大学生命科学学院 河南省功能微生物绿色转化技术工程实验室 河南 新乡 453007 |
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| 基金项目： | 河南师范大学博士科研启动经费(5101049170167)；河南省高等学校重点科研项目(17A180028) |
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| 摘 要： | CRISPR(Clustered regularly interspaced short palindromic repeats)/Cas(CRISPR associated proteins)系统是在细菌和古生菌中发现的一种RNA指导的降解入侵病毒或质粒DNA的适应性免疫系统。由II型CRISPR/Cas系统改造而成的CRISPR/Cas9技术已经被开发成一种强大的基因组编辑和表达调控工具,并且广泛应用于基因功能研究、代谢工程和合成生物学等领域。本文从CRISPR/Cas9系统的发现过程、分类、作用原理、在微生物研究中的应用进展等方面进行总结,并展望了该技术的应用前景。
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| 关 键 词： | CRISPR/Cas9，基因组编辑，表达调控 |
Application progress of CRISPR/Cas9 technology in microbiological research |
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| ZHANG Kun,CHEN Jing-Chao,LI Yi,LIU Lei and WANG Hai-Lei.Application progress of CRISPR/Cas9 technology in microbiological research[J].Microbiology,2018,45(2):451-464. |
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| Authors: | ZHANG Kun CHEN Jing-Chao LI Yi LIU Lei and WANG Hai-Lei |
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| Institution: | Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China,Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China,Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China,Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China and Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China |
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| Abstract: | CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated proteins) is an acquired immune system found in bacteria and archaea that degrade target sequences of DNA from invading viruses or plasmids in a RNA-guided manner. CRISPR/Cas9 technology modified from type II CRISPR/Cas system has been developed as a strong genome editing and expression regulation tool, and widely used for studying gene function, metabolic engineering, synthetic biology, and other related fields. In this review, we summarize recent progress in development, classification, functional principle, applications and prospects of CRISPR/Cas9 technology in microbes. |
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| Keywords: | CRISPR/Cas9 Genome editing Expression regulation |
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