| 高灵敏假单胞菌铁载体的平板检测方法 |
| |
| 引用本文： | 陈绍兴,赵翔,沈萍,谢志雄.高灵敏假单胞菌铁载体的平板检测方法[J].微生物学通报,2006,33(3):122-127. |
| |
| 作者姓名： | 陈绍兴 赵翔 沈萍 谢志雄 |
| |
| 作者单位： | 武汉大学生命科学学院,武汉,430072 |
| |
| 基金项目： | 武汉市科学与技术晨光计划资助项目（No.20015005051） |
| |
| 摘 要： | CAS蓝色检测平板是一种筛选、检测各类细菌铁载体的常用方法，而蔗糖-天冬酰氨培养基被用于假单胞菌产铁载体规律的研究。用天冬氨酸替代天冬酰氨，将CAS蓝色检测液与蔗糖-天冬氨酸培养基（MSA培养基）相结合，得到一种改进的MSA-CAS检测平板。通过对假单胞菌属7个种8个株进行荧光与非荧光铁载体检测方面的比较研究，结果表明MSA-CAS检测平板假单胞菌铁载体的检测灵敏度比通用CAS检测平板高，而且在检测荧光铁载体方面具有荧光背景低、荧光铁载体晕圈明显和晕圈与背景的对比度大的优点。
|
| 关 键 词： | 铁载体 CAS检测平板 假单胞菌 MSA培养基 |
| 文章编号： | 0253-2654（2006）03-0122-06 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005-10-23 |
High-sensitive Detection Method for Siderophores from Pseudomonas |
| |
| CHEN Shao-Xing,ZHAO Xiang,SHEN Ping,XIE Zhi-Xiong.High-sensitive Detection Method for Siderophores from Pseudomonas[J].Microbiology,2006,33(3):122-127. |
| |
| Authors: | CHEN Shao-Xing ZHAO Xiang SHEN Ping XIE Zhi-Xiong |
| |
| Institution: | College of Life Sciences, Wuhan University, Wuhan 430072 |
| |
| Abstract: | CAS (Chrome azurol S) assay was a universal method for detecting the bacterial siderophores, and Sugar-Asn liquid medium has been applied to the studies on siderophores from Pieudomonas. In this paper, Asp has been substituted for Asn, and MSA-CAS agar plate was developed by integrating the MSA (sugar-Asp) medium and CAS bright blue dye, which has been used in the universal CAS assay. On the aspect of siderophores detection , 8 strains of 7 species from Pseudomonas had been screened on MSA-CAS agar plates and universal CAS assay respectively. The results showed that MSA-CAS agar was higher-sensitive and lower basic fluorescent than universal CAS assay. |
| |
| Keywords: | Siderophore CAS assay Pseudomonas MSA medium |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
